The effects of isoimperatorin isolated from Agelicae dahuricae on cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells

被引：7

作者：

Tae Chul Moon

Meihua Jin

Jong Keun Son

Hyeun Wook Chang

机构：

[1] University of Alberta,Pulmonary Research Group, Department of Medicine

[2] Yeungnam University,College of Pharmacy

来源：

Archives of Pharmacal Research | 2008年 / 31卷

关键词：

Isoimperatorin; Cyclooxygenase-1, 2, 5-Lipoxygenase; Bone marrow-derived mast cells;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Isoimperatorin (4-[(3-Methyl-2-butenyl)oxy]-7H-furo[3,2-g][1]benzopyran-7-one) is a medicinal herbal product that is isolated from the dried roots of Angelicae dahuricae. Isoimperatorin inhibits the cyclooxygenase-2 (COX-2) and COX-1-dependent phases of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner, with IC50 values of 10.7 μM and 24 μM, respectively. However, this compound was not able to inhibit COX-1 and 2 protein expression in BMMC that were treated with concentrations of up to 50 μM, which indicates that isoimperatorin directly inhibits COX-2 activity. Furthermore, this compound consistently inhibited the production of leukotriene C4 (LTC4), as well as the degranulation reaction in BMMC, with an IC50 value of 5.7 μM and 9 μM, respectively, and these effects occurred in a dose dependent fashion. These results demonstrate that isoimperatorin has a dual cyclooxygenase-2 selective/5-lipoxygenase inhibitory activity, and therefore may provide the basis for novel anti-inflammatory drugs.

引用

页码：210 / 215

页数：5

共 127 条

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