A comparison of dispersing media for various engineered carbon nanoparticles

被引：81

作者：

Buford M.C. ^{[1
]}

Hamilton Jr. R.F. ^{[1
]}

Holian A. ^{[1
]}

机构：

[1] Center for Environmental Health Sciences, Department of Biomedical and Pharmaceutical Sciences, University of Montana, Missoula, MT

来源：

Particle and Fibre Toxicology | / 4卷 / 1期

关键词：

Fetal Calf Serum; Carbon Nanoparticles; Large Agglomerate; Suspension Medium; Small Agglomerate;

D O I：

10.1186/1743-8977-4-6

中图分类号：

学科分类号：

摘要：

Background: With the increased manufacture and use of carbon nanoparticles (CNP) there has been increasing concern about the potential toxicity of fugitive CNP in the workplace and ambient environment. To address this matter a number of investigators have conducted in vitro and in vivo toxicity assessments. However, a variety of different approaches for suspension of these particles (culture media, Tween 80, dimethyl sulfoxide, phosphate-buffered saline, fetal calf serum, and others), and different sources of materials have generated potentially conflicting outcomes. The quality of the dispersion of nanoparticles is very dependent on the medium used to suspend them, and this then will most likely affect the biological outcomes. Results: In this work, the distributions of different CNP (sources and types) have been characterized in various media. Furthermore, the outcome of instilling the different agglomerates, or size distributions, was examined in mouse lungs after one and seven days. Our results demonstrated that CNP suspended in serum produced particle suspensions with the fewest large agglomerates, and the most uniform distribution in mouse lungs. In addition, no apparent clearance of instilled CNP took place from lungs even after seven days. Conclusion: This work demonstrates that CNP agglomerates are present in all dispersing vehicles to some degree. The vehicle that contains some protein, lipid or protein/lipid component disperses the CNP best, producing fewer large CNP agglomerates. In contrast, vehicles absent of lipid and protein produce the largest CNP agglomerates. The source of the CNP is also a factor in the degree of particle agglomeration within the same vehicle. © 2007 Buford et al; licensee BioMed Central Ltd.

引用

共 17 条

[1]

Oberdorster G., Maynard A., Donaldson K., Castranova V., Fitzpatrick J., Ausman K., Carter J., Karn B., Kreyling W., Lai D., Olin S., Monteiro-Riviere N., Warheit D., Yang H., Principles for characterizing the potential human health effects from exposure to nanomaterials: Elements of a screening strategy, Part Fibre Toxicol, 2, (2005)

[2]

Lam C.W., James J.T., McCluskey R., Arepalli S., Hunter R.L., A review of carbon nanotube toxicity and assessment of potential occupational and environmental health risks, Crit Rev Toxicol, 36, 3, pp. 189-217, (2006)

[3]

Lam C.W., James J.T., McCluskey R., Hunter R.L., Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation, Toxicol Sci, 77, 1, pp. 126-134, (2004)

[4]

Warheit D.B., Laurence B.R., Reed K.L., Roach D.H., Reynolds G.A., Webb T.R., Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats, Toxicol Sci, 77, 1, pp. 117-125, (2004)

[5]

Shvedova A.A., Kisin E.R., Mercer R., Murray A.R., Johnson V.J., Potapovich A.I., Tyurina Y.Y., Gorelik O., Arepalli S., Schwegler-Berry D., Hubbs A.F., Antonini J., Evans D.E., Ku B.K., Ramsey D., Maynard A., Kagan V.E., Castranova V., Baron P., Unusual inflammatory and fibrogenic pulmonary responses to single-walled carbon nanotubes in mice, Am J Physiol Lung Cell Mol Physiol, 289, 5, (2005)

[6]

Moore V.C., Stranto M.S., Haroz E.H., Hauge R.H., Smalley R.E., Scmidt J., Talmon Y., Individually suspended single-walled carbon nanotubes in various surfactants, Nano Letters, 3, 10, pp. 1379-1382, (2003)

[7]

Monteiro-Riviere N.A., Nemanich R.J., Inman A.O., Wang Y.Y., Riviere J.E., Multi-walled carbon nanotube interactions with human epidermal keratinocytes, Toxicol Lett, 155, 3, pp. 377-384, (2005)

[8]

Jia G., Wang H., Yan L., Wang X., Pei R., Yan T., Zhao Y., Guo X., Cytotoxicity of carbon nanomaterials: Single-wall nanotube, multi-wall nanotube, and fullerene, Environ Sci Technol, 39, 5, pp. 1378-1383, (2005)

[9]

Shvedova A.A., Castranova V., Kisin E.R., Schwegler-Berry D., Murray A.R., Gandelsman V.Z., Maynard A., Baron P., Exposure to carbon nanotube material: Assessment of nanotube cytotoxicity using human keratinocyte cells, J Toxicol Environ Health A, 66, 20, pp. 1909-1926, (2003)

[10]

Cherukuri P., Bachilo S.M., Litovsky S.H., Weisman R.B., Near-infrared fluorescence microscopy of single-walled carbon nanotubes in phagocytic cells, J Am Chem Soc, 126, 48, pp. 15638-15639, (2004)

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