A rapid and simple method for small-scale DNA extraction in Agavaceae and other tropical plants

被引：9

作者：

Keb-Llanes M. ^{[1
]}

González G. ^{[1
,2
]}

Chi-Manzanero B. ^{[1
]}

Infante D. ^{[1
,3
]}

机构：

[1] Unidad de Biotecnología, Ctro. de Invest. Cie. de Yucatan, Mérida, Yucatán

[2] Universidad de Matanzas, Matanzas

[3] Instituto de Estudios Avanzados-IDEA, Centro de Biotecnología, Caracas 10151-A, Apdo. 17606, Parque Central

来源：

Plant Molecular Biology Reporter | 2002年 / 20卷 / 3期

关键词：

AFLP; Agave fourcroydes; Genomic DNA; Henequen;

D O I：

10.1007/BF02782465

中图分类号：

学科分类号：

摘要：

This protocol permits the simultaneous extraction of clean DNA from many samples with little reagent waste, thus decreasing the cost of analysis per sample. The procedure is rapid, permitting the processing of 80-100 samples per day. Using this protocol, we analyzed naturally propagated and micropropagated populations of henequen and other Agavaceae species using amplified fragment length polymorphism (AFLP). Agaves have succulent leaves with a content that is high in fiber and chemical compounds. Therefore, this protocol should work for other tropical and subtropical plant species. The protocol involves precipitation and resuspension of DNA 3 times at the end of the preparation; this increases DNA digestibility and the sharpness of AFLP bands.

引用

页码：299a / 299e

共 10 条

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