Actinomycetales bacteria from a spruce stand: Characterization and effects on growth of root symbiotic and plant parasitic soil fungi in dual culture

被引:51
作者
Andreas Maier
Julia Riedlinger
Hans-Peter Fiedler
Rüdiger Hampp
机构
[1] University of Tübingen,Department of Botany
[2] University of Tübingen,Department of Microbiology
关键词
Fine Root; Bacterial Isolate; Fruiting Body; Soil Fungus; Wood Decay;
D O I
10.1007/s11557-006-0083-y
中图分类号
学科分类号
摘要
The rhizosphere, the narrow zone of soil around living roots, is characterized by root exudates which attract soil microorganisms. Most importantly, certain soil fungi establish symbiotic interactions with fine roots which enhance nutrient availability for the plant partner (mycorrhiza). The establishment of such a symbiosis can be affected by soil bacteria. In this study we isolated Gram-positive soil bacteria from the rhizosphere of a spruce stand rich with fly agaric (Amanita muscaria) fruiting bodies. Using a coculture technique in Petri dishes, bacterial isolates were characterized by their effect on the growth of fungal hyphae. A group of bacterial strains were identified which significantly promoted growth of fly agaric hyphae. One of these strains was shown to additionally inhibit growth of pathogenic fungi such as Armillaria obscura (wide host range) and Heterobasidion annosum (causes wood decay in conifers). Taxonomic characterization of the effective bacterial isolates by their morphological appearance, by the analysis of diaminopimelic acid, cell wall sugars, and DNA sequencing (16S rDNA) identified them as actinomycetes, some of which are not yet contained in data banks.
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页码:129 / 136
页数:7
相关论文
共 86 条
[1]  
Bending G.D.(2002)Characterisation of bacteria from FEMS Microbiology Ecolology 39 219-227
[2]  
Poole E.J.(1956) mycorrhizas and their effects on root-fungus interactions and plant growth Journal of General Microbiology 14 583-600
[3]  
Whipps J.M.(1998)The chemical composition of the cell wall in some Gram-positive bacteria and its possible value as a taxonomic character Plant and Soil 201 241-249
[4]  
Read D.J.(2002)Effects of bacteria on mycorrhizal development and growth of container grown New Phytolologist 153 81-89
[5]  
Cummins C.S.(1997) F. Muell. seedlings Applied and Environmental Microbiology 63 139-144
[6]  
Harris H.(1994)Mycorrhiza helper bacteria stimulate ectomycorrhizal symbiosis of New Phytolologist 128 197-210
[7]  
Dunstan W.A.(1995) with Canadian Journal of Microbiology 41 109-117
[8]  
Malajczuk N.(1999)Location and survival of Mycorrhiza helper Applied and Environmental Microbiology 65 1428-1434
[9]  
Dell B.(1904) during establishment of ectomycorrhizal symbiosis between Arbeiten der Deutschen Landwirtschaftlichen Gesellschaft 98 59-78
[10]  
Founoune H.(1983) and Douglas Fir Applied and Environmental Microbiology 46 925-929