Caldesmon-dependent switching between capillary endothelial cell growth and apoptosis through modulation of cell shape and contractility

被引：49

作者：

Yasushi Numaguchi

Sui Huang

Thomas R. Polte

Gabriel S. Eichler

Ning Wang

Donald E. Ingber

机构：

[1] Vascular Biology Program, Department of Pathology, Children's Hosp./Harvard Med. Sch., Boston, MA

[2] Physiology Program, Harvard School of Public Health, Boston, MA

[3] Vascular Biology Program, Department of Surgery, Children's Hosp./Harvard Med. Sch., Boston, MA 02115

来源：

Angiogenesis | 2003年 / 6卷 / 1期

关键词：

Cell cycle; Cytoskeleton; Extracellular matrix; Mechanical force; Microfilament; Tension;

D O I：

10.1023/A:1025821517679

中图分类号：

学科分类号：

摘要：

Caldesmon (CaD), a protein component of the actomyosin filament apparatus, modulates cell shape and cytoskeletal structure when overexpressed. When capillary endothelial cells were infected with an adenoviral vector encoding GFP-CaD under Tet-Off control, progressive inhibition of contractility, loss of actin stress fibers, disassembly of focal adhesions, and cell retraction resulted. This was accompanied by a cell shape (rounding)-dependent increase in apoptosis and concomitant inhibition of cell cycle progression. Cell growth also was inhibited in low expressor cells in which cell tension was suppressed independently of significant changes in cell shape, cytoskeletal structure, or focal adhesions. Thus, changes in both cytoskeletal structure and contractility appear to be central to the mechanism by which extracellular matrix-dependent changes in capillary cell shape influence growth and apoptosis during angiogenesis, and hence the cytoskeleton may represent a potential target for anti-angiogenesis therapy.

引用

页码：55 / 64

页数：9

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