A nucleolar localizing Rev binding element inhibits HIV replication

被引：21

作者：

Michienzi A. ^{[1
,3
]}

De Angelis F.G. ^{[2
]}

Bozzoni I. ^{[2
]}

Rossi J.J. ^{[1
,4
]}

机构：

[1] Division of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010

[2] Istituto Pasteur, Department of Genetics and Molecular Biology, University of Rome La Sapienza, Rome

[3] Istituto Superiore di Sanita, Department of Cell Biology and Neuroscience, 00161 Rome

[4] Division of Molecular Biology, Graduate School of Biological Sciences, Beckman Research Institute of the City of Hope, Duarte, CA 91010

来源：

AIDS Research and Therapy | / 3卷 / 1期

关键词：

Long Terminal Repeat; eGFP Expression; Human Immunodeficiency Virus Replication; Colony Form Unit Assay; Normal Human Bone Marrow;

D O I：

10.1186/1742-6405-3-13

中图分类号：

学科分类号：

摘要：

The Rev protein of the human immunodeficiency virus (HIV) facilitates the nuclear export of intron containing viral mRNAs allowing formation of infectious virions. Rev traffics through the nucleolus and shuttles between the nucleus and cytoplasm. Rev multimerization and interaction with the export protein CRM1 takes place in the nucleolus. To test the importance of Rev nucleolar trafficking in the HIV-1 replication cycle, we created a nucleolar localizing Rev Response Element (RRE) decoy and tested this for its anti-HIV activity. The RRE decoy provided marked inhibition of HIV-1 replication in both the CEM T-cell line and in primary CD34+ derived monocytes. These results demonstrate that titration of Rev in the nucleolus impairs HIV-1 replication and supports a functional role for Rev trafficking in this sub-cellular compartment. © 2006 Michienzi et al; licensee BioMed Central Ltd.

引用

共 26 条

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