A simple system for pea transformation

被引：80

作者：

Bean S.J. ^{[1
]}

Gooding P.S. ^{[1
]}

Mullineaux P.M. ^{[1
]}

Davies D.R. ^{[1
]}

机构：

[1] John Innes Centre, Norwich Research Park, Colney, Norwich

来源：

Plant Cell Reports | 1997年 / 16卷 / 8期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Agrobacterium tumefaciens; Bar gene; Cotyledonary meristem; Pea transformation; Phosphinothricin resistance;

D O I：

10.1007/BF01142315

中图分类号：

学科分类号：

摘要：

The lateral cotyledonary meristems of germinating Pisum sativum cv. Puget seeds were used to develop a reproducible Agrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion.

引用

页码：513 / 519

页数：6

共 25 条

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