Anti-oxidant effect of gold nanoparticles restrains hyperglycemic conditions in diabetic mice

被引：275

作者：

BarathManiKanth S. ^{[1
]}

Kalishwaralal K. ^{[1
]}

Sriram M. ^{[1
]}

Pandian S.R.K. ^{[1
]}

Youn H.-S. ^{[2
]}

Eom S. ^{[2
]}

Gurunathan S. ^{[1
]}

机构：

[1] Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University, Krishnankoil-626190, Tamilnadu, Anand Nagar

[2] School of Life Sciences, Systems Biology Research Center, Gwangju Institute of Science and Technology

来源：

Journal of Nanobiotechnology | / 8卷 / 1期

关键词：

Gold Nanoparticles; Inductively Couple Plasma Mass Spectrometry; Reactive Oxygen Species Generation; Diabetic Mouse; Diabetic Control;

D O I：

10.1186/1477-3155-8-16

中图分类号：

学科分类号：

摘要：

Background: Oxidative stress is imperative for its morbidity towards diabetic complications, where abnormal metabolic milieu as a result of hyperglycemia, leads to the onset of several complications. A biological antioxidant capable of inhibiting oxidative stress mediated diabetic progressions; during hyperglycemia is still the need of the era. The current study was performed to study the effect of biologically synthesized gold nanoparticles (AuNPs) to control the hyperglycemic conditions in streptozotocin induced diabetic mice.Results: The profound control of AuNPs over the anti oxidant enzymes such as GSH, SOD, Catalase and GPx in diabetic mice to normal, by inhibition of lipid peroxidation and ROS generation during hyperglycemia evidence their anti-oxidant effect during hyperglycemia. The AuNPs exhibited an insistent control over the blood glucose level, lipids and serum biochemical profiles in diabetic mice near to the control mice provokes their effective role in controlling and increasing the organ functions for better utilization of blood glucose. Histopathological and hematological studies revealed the non-toxic and protective effect of the gold nanoparticles over the vital organs when administered at dosage of 2.5 mg/kilogram.body.weight/day. ICP-MS analysis revealed the biodistribution of gold nanoparticles in the vital organs showing accumulation of AuNPs in the spleen comparatively greater than other organs.Conclusion: The results obtained disclose the effectual role of AuNPs as an anti-oxidative agent, by inhibiting the formation of ROS, scavenging free radicals; thus increasing the anti-oxidant defense enzymes and creating a sustained control over hyperglycemic conditions which consequently evoke the potential of AuNPs as an economic therapeutic remedy in diabetic treatments and its complications. © 2010 BarathManiKanth et al; licensee BioMed Central Ltd.

引用

共 69 条

[1]

Aylward G.W., Progressive changes in diabetics and their management, Eye, 19, 10, pp. 1115-1118, (2005)

[2]

Wild S., Roglic G., Green A., Sicree R., King H., Global prevalence of diabetes, Diabetes Care, 27, pp. 1047-1053, (2004)

[3]

Aronson D., Hyperglycemia and pathobiology of diabetic complications, Adv Cardiol, 45, pp. 1-16, (2008)

[4]

Harrison D., Griendling K.K., Landmesser U., Hornig B., Drexler H., Role of oxidative stress in atherosclerosis, Am J Cardiol, 91, 3 A, (2003)

[5]

Dash D., Shrivastava S., Applying Nanotechnology to Human Health: Revolution in Biomedical Sciences, J Nanotechnology

[6]

Norton S., A brief history of potable gold, Mol Interv, 8, 3, pp. 120-125, (2008)

[7]

Jeon K.I., Byun M.S., Jue D.M., Gold compound auranofin inhibits Ikappa B kinase (IKK) by modifying Cys-179 of IKKbeta subunit, Exp Mol Med, 35, pp. 61-66, (2003)

[8]

Kim N.H., Lee M.Y., Park S.J., Choi J.S., Oh M.K., Kim I.S., Auranofin blocks interleukin-6 signalling by inhibiting phosphorylation of JAK1 and STAT3, Immunology, 122, pp. 607-614, (2007)

[9]

Shah Z.A., Vohora S.B., Antioxidant/Restorative Effects of Calcined Gold Preparations Used in Indian Systems of Medicine against Global and Focal Models of Ischaemia, Pharmacol Toxicol, 90, pp. 254-259, (2002)

[10]

Kalishwaralal K., Deepak V., Pandian S.R.K., Gurunathan S., Biological synthesis of gold nanocubes using Bacillus licheniformis, Bioresour Technol, 100, pp. 5356-5358, (2009)

← 1 2 3 4 5 6 7 →