Bacteriophage OP1, lytic for Xanthomonas oryzae pv. oryzae, changes its host range by duplication and deletion of the small domain in the deduced tail fiber gene

被引：55

作者：

Inoue Y. ^{[1
]}

Matsuura T. ^{[1
]}

Ohara T. ^{[2
]}

Azegami K. ^{[1
]}

机构：

[1] National Agricultural Research Center

[2] Yokohama Plant Protection Station, Yokohama

来源：

Journal of General Plant Pathology | 2006年 / 72卷 / 2期

关键词：

Bacteriophage OP[!sub]1[!/sub; Genetic modification; Genome analysis; HNH-family gene; Host range; Tail fiber gene;

D O I：

10.1007/s10327-005-0252-x

中图分类号：

学科分类号：

摘要：

The entire genome of bacteriophage OP1, lytic for Xanthomonas oryzae pv. oryzae causing bacterial leaf blight of rice, and the partial genomes of related phages were sequenced and analyzed. The OP1 genome comprises double-stranded, 4785-bp long DNA with 51.1% G + C content. Fifty-nine open reading frames (ORFs) were detected. ORF25 had similarity with the tail fiber gene of phages, whose product is related to host specificity. The ORF25 regions were amplified from four host-range mutants (OP1h, OP 1hC, OP1h2, and OP1h2C) by polymerase chain reaction, and their deduced amino acid sequences were compared. Three mutants (OP1hC, OP1h2, OP1h2C) had duplications of a small domain in the N-terminal portion, although there were slight differences in the position of the duplicated sequences. One mutant OP1h had substituted amino acids in the duplication region. New mutants isolated in the laboratory (OP1hC and OP1h2C from OP1 and OP1h2) acquired the ability to lyse strain N5874 belonging to phagovar (lysotype) C. However, they rapidly lost this lytic ability when incubated with other phagovars. This loss was always accompanied by a loss of the characteristic repeats, suggesting that the host range of OP 1-related phages changed mainly through duplication and deletion of a small domain in ORF25. © The Phytopathological Society of Japan and Springer-Verlag 2006.

引用

页码：111 / 118

页数：7

共 25 条

[1]

Aravind L., Makarova K.S., Koonin E.V., Survey and summary: Holliday junction resolvases and related nucleases: Identification of new families, phyletic distribution and evolutionary trajectories, Nucleic Acids Res, 28, pp. 3417-3432, (2000)

[2]

Ezuka A., Kaku H., A historical review of bacterial blight of rice, Bull Natl Inst Agrobiol Resour, 15, pp. 61-74, (2000)

[3]

Goodridge L.D., Bacteriophage biocontrol of plant pathogens: Fact or fiction?, Trends Biotechnol, 22, pp. 384-385, (2004)

[4]

Haggard-Ljungquist E., Halling C., Calendar R., DNA sequences of the tail fiber genes of bacteriophage P2: Evidence for horizontal transfer of tail fiber genes among unrelated bacteriophages, J Bacteriol, 174, pp. 1462-1477, (1992)

[5]

Hoess R., Wierzbicki A., Abremski K., Isolation and characterization of intermediates in site-specific recombination, Proc Natl Acad Sci USA, 84, pp. 6840-6844, (1987)

[6]

Hsu P.L., Landy A., Resolution of synthetic att-site Holliday structures by the integrase protein of bacteriophage lambda, Nature, 311, pp. 721-726, (1984)

[7]

Ikari H., Wakimoto S., Properties of a new Xanthomonas oryzae phage (OP<sub>1h</sub>) isolated at the Kyushu National Agricultural Experiment Station, Proc Assoc Plant Prot Kyushu, 4, pp. 38-40, (1958)

[8]

Inoue Y., Matsuura T., Ohara T., Azegami K., Sequence analysis of the genome of OP<sub>2</sub>, a lytic bacteriophage of Xanthomonas oryzae pv. oryzae, J Gen Plant Pathol, 172, pp. 104-110, (2006)

[9]

Kim P.V., Wakimoto S., Some properties of Xanthomonas campestris pv. oryzae and its phages from Vietnam, Ann Phytopathol Soc Jpn, 38, pp. 68-74, (1982)

[10]

Kuo T.-T., Huang T.-C., Wu R.-Y., Yang C.-M., Characterization of three bacteriophages of Xanthomonas oryzae (Uyeda et Ishiyama) Dowson, Bot Bull Acad Sin, 8, pp. 246-254, (1967)

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