Hepatocyte and keratinocyte growth factors and their receptors in human lung emphysema

被引：13

作者：

Bonay M. ^{[1
,2
]}

Boutten A. ^{[1
,3
]}

Leçon-Malas V. ^{[3
]}

Marchal J. ^{[1
]}

Soler P. ^{[1
]}

Fournier M. ^{[5
]}

Leseche G. ^{[6
]}

Dehoux M. ^{[1
,3
]}

Cretani B. ^{[1
,4
]}

机构：

[1] INSERM U 700, Hôpital Bichat-Claude Bernard, Université Paris 7, 75722 Paris Cedex 18

[2] Service de Physiologie-Explorations Fonctionnelles, Hôpital Bichat-Claude Bernard, Université Paris 7, 75722 Paris Cedex 18

[3] Service de Biochimie A, Hôpital Bichat-Claude Bernard, Université Paris 7, 75722 Paris Cedex 18

[4] Service de Pneumologie, Hôpital Bicht-Claude Bernard, Université Paris 7, 75722 Paris Cedex 18

[5] Service de Pneumologie, Hôpital Beaujon, Université Paris 7, Paris

[6] Service de Chirurgie Thoracique, Hôpital Beaujon, Université Paris 7, Paris

来源：

BMC Pulmonary Medicine | / 5卷 / 1期

关键词：

Hepatocyte Growth Factor; Airflow Obstruction; Keratinocyte Growth Factor; Lung Homogenate; Lung Emphysema;

D O I：

10.1186/1471-2466-5-13

中图分类号：

学科分类号：

摘要：

Background: Hepatocyte and keratinocyte growth factors are key growth factors in the process of alveolar repair. We hypothesized that excessive alveolar destruction observed in lung emphysema involves impaired expression of hepatocyte and keratinocyte growth factors or their respective receptors, c-met and keratinocyte growth factor receptor. The aim of our study was to compare the expression of hepatocyte and keratinocyte growth factors and their receptors in lung samples from 3 groups of patients: emphysema; smokers without emphysema and non-smokers without emphysema. Methods: Hepatocyte and keratinocyte growth factor proteins were analysed by immunoassay and western blot; mRNA expression was measured by real time quantitative polymerase chain reaction. Results: Hepatocyte and keratinocyte growth factors, c-met and keratinocyte growth factor receptor mRNA levels were similar in emphysema and non-emphysema patients. Hepatocyte growth factor mRNA correlated negatively with FEV1 and the FEV1/FVC ratio both in emphysema patients and in smokers with or without emphysema. Hepatocyte and keratinocyte growth factor protein concentrations were similar in all patients' groups. Conclusions: The expression of hepatocyte and keratinocyte growth factors and their receptors is preserved in patients with lung emphysema as compared to patients without emphysema. Hepatocyte growth factor mRNA correlates with the severity of airflow obstruction in smokers. © 2005 Bonay et al., licensee BioMed Central Ltd.

引用

页数：27

共 31 条

[1]

Barnes P.J., Chronic obstructive pulmonary disease, N. Engl. J. Med., 343, pp. 269-280, (2000)

[2]

Shapiro S.D., The macrophage in chronic obstructive pulmonary disease, Am. J. Respir. Crit. Care Med., 160, (1999)

[3]

Finkelstein R., Fraser R.S., Ghezzo H., Cosio M.G., Alveolar inflammation and its relation to emphysema in smokers, Am. J. Respir. Crit. Care Med., 152, pp. 1666-1672, (1995)

[4]

Retamales I., Elliott W.M., Meshi B., Coxson H.O., Pare P.D., Sciurba F.C., Rogers R.M., Hayashi S., Hogg J.C., Amplification of inflammation in emphysema and its association with latent adenoviral infection, Am. J. Respir. Crit. Care Med., 164, pp. 469-473, (2001)

[5]

Turato G., Zuin R., Miniati M., Baraldo S., Rea F., Beghe B., Monti S., Formichi B., Boschetto P., Harari S., Papi A., Maestrelli P., Fabbri L.M., Saetta M., Airway inflammation in severe chronic obstructive pulmonary disease: Relationship with lung function and radiologic emphysema, Am. J. Respir. Crit. Care Med., 166, pp. 105-110, (2002)

[6]

Kasahara Y., Tuder R.M., Taraseviciene-Stewart L., Le Cras T.D., Abman S., Hirth P.K., Waltenberger J., Voelkel N.F., Inhibition of VEGF receptors causes lung cell apoptosis and emphysema, J. Clin. Invest., 106, pp. 1311-1319, (2000)

[7]

Kasahara Y., Tuder R.M., Cool C.D., Lynch D.A., Flores S.C., Voelkel N.F., Endothelial cell death and decreased expression of vascular endothelial growth factor and vascular endothelial growth factor receptor 2 in emphysema, Am. J. Respir. Crit. Care Med., 163, pp. 737-744, (2001)

[8]

Aoshiba K., Yokohori N., Nagai A., Alveolar wall apoptosis causes lung destruction and emphysematous changes, Am. J. Respir. Cell Mol. Biol., 28, pp. 555-562, (2003)

[9]

Mason R.J., Leslie C.C., McCormick-Shannon K., Deterding R.R., Nakamura T., Rubin J.S., Shannon J.M., Hepatocyte growth factor is a growth factor for rat alveolar type II cells, Am. J. Respir. Cell Mol. Biol., 11, pp. 561-567, (1994)

[10]

Ohmichi H., Matsumoto K., Nakamura T., In vivo mitogenic action of HGF on lung epithelial cells: Pulmotrophic role in lung regeneration, Am. J. Physiol., 270, (1996)

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