Characterization of the putative α subunit of a heterotrimeric G protein in rice

被引：3

作者：

Yukimoto Iwasaki

Teruhisa Kato

Toshio Kaidoh

Atsushi Ishikawa

Tadashi Asahi

机构：

[1] Fukui Prefectual University,Department of Bioscience, Faculty of Biotechnology

来源：

Plant Molecular Biology | 1997年 / 34卷

关键词：

ADP ribosylation; α subunit; cholera toxin; heterotrimeric G protein; rice; signal transduction;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A recombinant protein with a cDNA that encodes the putative α subunit of a rice heterotrimeric G protein was synthesized in Escherichia coli and purified. The recombinant protein (rGrice α) with an apparent molecular mass of 45 kDa was bound with guanosine 5′-(3-O-thio)triphosphate with an apparent association constant (kapp) of 0.36. The protein also hydrolyzed GTP and its Kcat was 0.44. rGrice α was ADP-ribosylated by activated cholera toxin.

引用

页码：563 / 572

页数：9

共 110 条

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