Electron microscopic stereology of capillary endothelial cells and cardiomyocytes in artificially arrested canine hearts

被引：6

作者：

Schmiedl A. ^{[1
]}

Schnabel P.A. ^{[2
]}

Marten K. ^{[1
]}

Von Schmeling H.K.B. ^{[3
]}

Richter J. ^{[1
]}

机构：

[1] Department of Anatomy, Division of Electron Microscopy, 37075 Göttingen

[2] Department of Pathology, Heidelberg, Im Neuenheimer Feld

[3] Department of Surgery, Central Hospital Bremen-Nord, Bremen

来源：

Medical Electron Microscopy | 1999年 / 32卷 / 3期

关键词：

Capillaries; Cardioplegic solutions; Endothelium; Heart; Stereology; Ultrastructure;

D O I：

10.1007/s007950050022

中图分类号：

学科分类号：

摘要：

In open heart surgery and transplantation, sufficient structural preservation of the myocardium immediately following cardioplegic arrest is a precondition for overcoming ischemia and for resumption of postischemic function. Therefore, we compared the protective effect of three clinically applied cardioplegic solutions with fibrillating and beating hearts using structural criteria. Left ventricular samples were taken from (1) beating, or (2) fibrillating or arrested hearts following coronary perfusion with (3) St. Thomas' Hospital solution, (4) histidine tryptophane ketoglutalate (HTK) (Custodiol), or (5) University of Wisconsin (UW) solution and fixed by immersion. Ultrastructural differences in the swelling of capillary endothelial cells and myocytes were quantitatively evaluated using stereological methods. Endothelial cells were somewhat more swollen after St. Thomas perfusion than those in beating and fibrillating hearts. HTK-arrested hearts showed significantly lower values for cellular edema than beating hearts. UW perfusion resulted in the (significantly) lowest degree of endothelial cell edema. Edematous changes in myocytes were significantly greater in St. Thomas-arrested hearts than in UW- or HTK-arrested hearts. Cardiomyocyte edema in beating and fibrillating hearts was comparable to that in St. Thomas-perfused hearts. Thus, the stereological analysis revealed significant differences between cardioplegic solutions in structural preservation of myocardial ultrastructure.

引用

页码：151 / 160

页数：9