PCR detection of Listeria monocytogenes in different food products compared with the mini-VIDAS LMO system and the standard procedure ISO 11290-1

被引：9

作者：

Aznar R. ^{[1
,2
,4
]}

Solís I. ^{[3
]}

机构：

[1] Departamento de Microbiología Y Ecología, Universitat de València

[2] Instituto de Agroquímica Y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas

[3] Departamento de Biotecnología, Instituto de Agroquímica Y Tecnología de Los Alimentos, E-46100 Burjassot, Valencia

来源：

Journal für Verbraucherschutz und Lebensmittelsicherheit | 2006年 / 1卷 / 2期

关键词：

Food; Listeria monocytogenes; mini-VIDAS; PCR detection;

D O I：

10.1007/s00003-006-0019-0

中图分类号：

学科分类号：

摘要：

The presence of Listeria monocytogenes in 225 natural samples, including different food types, was investigated by three methods: (i) culture-based standard procedure ISO 11290-1, (ii) mini-VIDAS (Vitek Immuno Diagnostic Assay System) LMO, an enzyme linked fluorescent assay (ELFA) commercially available, and (iii) PCR using a previously established procedure. Identification of isolates recovered from the standard method and mini-VIDAS, on Oxford and PALCAM selective plates, was carried out using the API-Lis system and also by PCR, using L. monocytogenes specific primers. In all, 65 samples (64 meat products and one smoked salmon) were positive with any of the three procedures assayed. Taking into account the results based on PCR identification, the standard culture-based method found 22 and the mini-VIDAS 23, while PCR detected 60 positive samples in a total of 225 food samples. For comparative purposes, a set of "known positive samples" was established as reference that included 33 natural samples from which L. monocytogenes isolates (identified by specific PCR) had been recovered. The mini-VIDAS and ISO 11290-1 methods were equally sensitive. Compared to them PCR was clearly the more accurate and efficient procedure for detection of L. monocytogenes in food. Moreover, it showed no false negative results. The PCR approach can be completed in 48 working hours, and because of its specificity might eventually be cheaper than the other two procedures. Consequently we recommend PCR for routine detection of L. monocytogenes in food. © Birkhäuser Verlag, 2006.

引用

页码：115 / 120

页数：5

共 34 条

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