Transformation of wheat (Triticum aestivum L.) microspore-derived callus and microspores by particle bombardment

被引：34

作者：

Foiling L. ^{[1
]}

Olesen A. ^{[1
]}

机构：

[1] Department of Agricultural Sciences, Plant Breeding and Crop Science, Royal Veterinary/Agricultural Univ., 871 Frederiksberg C

来源：

Plant Cell Reports | 2001年 / 20卷 / 07期

关键词：

Microspore culture; Microspore-derived callus; Particle bombardment; Transformation; Wheat;

D O I：

10.1007/s002990100371

中图分类号：

学科分类号：

摘要：

Twenty-seven bialaphos-tolerant and GUS-positive lines were produced from 2,940 callus pieces after particle bombardment of wheat microspore-derived callus. Regenerated plants were mainly of the albino type. In an attempt to avoid this problem, wheat microspores were used as target cells for particle bombardment. Pre-cultivation for a period of 3-8 days improved the frequency of GUS-expressing microspores. Helium rupture pressures between 1,100 psi and 1,800 psi, the amount of gold per bombardment (ranging from 37 μg to 300 μg) and particle size (0.6-1.0 μg) did not significantly affect transient expression. Microspore response measured as number of recovered embryos was not signifi- cantly affected by variations in helium pressure or amount of gold used, but response was significantly influenced by particle size. The highest number of GUS-expressing embryos was 3.5 embryos per 106 microspores, which was obtained after 4 days of pre-cultivation, 1,350 psi rupture pressure, 0.6+1.0 μm particles (1:1) and 150 μg gold particles per bombardment.

引用

页码：629 / 636

页数：7

共 52 条

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