Molecular typing of erythromycin-resistant Streptococcus pyogenes strains with the M phenotype isolated in Italy

被引：16

作者：

Valisena S. ^{[1
]}

Falci C. ^{[1
]}

Mazzariol A. ^{[1
]}

Cornaglia G. ^{[1
]}

Cocuzza C.E. ^{[2
]}

Nicoletti P. ^{[3
]}

Rescaldani R. ^{[4
]}

Fontana R. ^{[1
]}

机构：

[1] Institute of Microbiology, University of Verona, Strada Le Grazie 8

[2] Institute of Medical Microbiology, University of Milan, Via Pascal 36

[3] Microbiology Laboratory, Careggi Hospital

[4] Microbiology Laboratory, San Gerardo Hospital

来源：

European Journal of Clinical Microbiology and Infectious Diseases | 1999年 / 18卷 / 4期

关键词：

Erythromycin; Macrolide Resistance; Erythromycin Resistance; PFGE Pattern; Sodium Arsenate;

D O I：

10.1007/s100960050274

中图分类号：

学科分类号：

摘要：

To assess the spread of the new M phenotype, various erythromycin-resistant Streptococcus pyogenes strains from three Italian cities (Verona, Monza, Florence) were characterised. Each strain was analysed for the presence of genes ermAM and mefA, for the ability to accumulate radioactive erythromycin in the absence of sodium arsenate, for the protein T serological type, and for the DNA macrorestriction profile identified by means of pulsed-field gel electrophoresis. In a number of strains, the presence of the inducible ermAM gene was demonstrated; all these strains were negative in the efflux-pump detection assay, did not possess the mefA gene, and had similar restriction profiles. The strains with the efflux mechanism and mefA gene belonged to different serotypes. Of these, only one serotype, T4, was isolated in all three cities. The restriction profile analysis with SmaI and SfiI revealed a very close correlation between strains with the same serotype.

引用

页码：260 / 264

页数：4

共 22 条

[1]

Cornaglia, G., Ligozzi, M., Mazzariol, A., Valentini, M., Orefici, G., Rapid increase of resistance to erythromycin and clindamycin in Streptococcus pyogenes in Italy, 1993-1995 (1996) Emerging Infectious Diseases, 2, pp. 339-342

[2]

Cocuzza, C., Mattina, R., Mazzariol, A., Orefici, G., Rescaldani, R., Primavera, A., Bramati, S., Fontana, R., High incidence of erythromycin-resistant Streptococcus pyogenes in Monza (North Italy) in untreated children with symptoms of acute pharyngo-tonsillitis: An epidemiologic and molecular study (1997) Microbiology and Drug Resistance, 3, pp. 371-378

[3]

Hsueh, P.R., Chen, H.M., Huang, A.Y., Wu, J.J., Decreased activity of erythromycin against Streptococcus pyogenes in Taiwan (1995) Antimicrobial Agents and Chemotherapy, 39, pp. 2239-2242

[4]

Seppälä, H., Nissinen, A., Jarvinen, H., Huovinen, S., Henrikson, T., Herva, E., Holm, S.E., Huovinen, P., Resistance to erythromycin in group A streptococci (1992) New England Journal of Medicine, 326, pp. 292-297

[5]

Seppälä, H., Nissinen, A., Yu, Q., Huovinen, P., Three different phenotypes of erythromycin-resistant S. Pyogenes in Finland (1993) Journal of Antimicrobial Chemotherapy, 32, pp. 885-891

[6]

Leclercq, R., Courvalin, P., Mechanisms of resistance to macrolide, lincosamides, and streptogramin antibiotics by target modification (1993) Antimicrobial Agents and Chemotherapy, 35, pp. 1267-1272

[7]

Weisblum, B., Erythromycin resistance by ribosome modification (1995) Antimicrobial Agents and Chemotherapy, 39, pp. 577-585

[8]

Sutcliffe, J., Tait-Kamradt, A., Wondrack, L., Streptococcus pneumoniae and Streptococcus pyogenes resistant to macrolides but sensitive to clindamycin: A common resistance pattern mediated by an efflux system (1996) Antimicrobial Agents and Chemotherapy, 40, pp. 1817-1824

[9]

Ruoff, K.L., Streptococcus (1995) Manual of Clinical Microbiology, pp. 299-307. , Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (eds). American Society for Microbiology, Washington, DC

[10]

(1997) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, , Approved standard M7-A4. NCCLS, Villanova, PA

← 1 2 3 →