Organotypic cocultures as skin equivalents: A complex and sophisticated in vitro system

被引：56

作者：

Stark H.-J. ^{[1
]}

Szabowski A. ^{[2
]}

Fusenig N.E. ^{[1
]}

Maas-Szabowski N. ^{[1
]}

机构：

[1] Div. of Carcinogenesis/Different., German Cancer Research Center, D-69120 Heidelberg

[2] Div. Signal Transduct./Growth Contr., German Cancer Research Center, Deutsches Krebsforschungszentrum, D-69120 Heidelberg

来源：

Biological Procedures Online | 2004年 / 6卷 / 1期

关键词：

Cocultures; Keratinocytes; Tissue engineering;

D O I：

10.1251/bpo72

中图分类号：

学科分类号：

摘要：

To assess the role of genes required for skin organogenesis, tissue regeneration and homeostasis, we have established in vitro skin equivalents composed of primary cells or cell lines, respectively. In these organotypic cocultures keratinocytes generate a normal epidermis irrespective of the species and tissue origin of fibroblasts. The combination of cells derived from mouse and human tissues facilitates the identification of the origin of compounds involved in epidermal tissue reconstitution and thus the precise analysis of growth regulatory mechanisms. © 2004. Biological Procedures Online.

引用

页码：55 / 60

页数：5

共 26 条

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[10]

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