Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60

被引：10

作者：

Wang L. ^{[1
]}

Zhao S. ^{[1
,2
]}

Wang H.-X. ^{[1
]}

Zou P. ^{[2
]}

机构：

[1] Department of Hematology, The Central Hospital of Wuhan

[2] Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

来源：

Frontiers of Medicine in China | 2010年 / 4卷 / 3期

关键词：

Bay; 11-7082; Fas/FasL system; HL-60; nuclear factor-kappa B;

D O I：

10.1007/s11684-010-0026-5

中图分类号：

学科分类号：

摘要：

This study explored the effects of nuclear factor-kappa B (NF-κB) inhibitor Bay 11-7082 on Fas/FasL system and Fas-mediated apoptosis in cell line HL-60 cells. The mRNA and protein levels of Fas, FasL, and X-linked inhibitor of apoptosis protein (XIAP) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FCM); the level of sFasL was evaluated by enzyme-linked immunosorbent assay (ELISA); and apoptosis was determined by FCM. After treatment with Bay 11-7082, the mRNA and protein levels of FasL and XIAP in HL-60 cells were significantly lower than in the controls (P < 0.05), but the mRNA and protein levels of Fas and sFasL did not change significantly (P > 0.05). Apoptotic rate of HL-60 cells treated with Bay 11-7082 was significantly higher than in the controls (P < 0.05). Therefore, we conclude that Bay 11-7082 can enhance Fas-mediated apoptosis in HL-60 cells by downregulating FasL and XIAP levels. © 2010 Higher Education Press and Springer-Verlag Berlin Heidelberg.

引用

页码：323 / 328

页数：5

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