A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications

被引：1

作者：

D.-H. Chen

P.C. Ronald

机构：

[1] University of California,Department of Plant Pathology

来源：

Plant Molecular Biology Reporter | 1999年 / 17卷

关键词：

CTAB; DNA extraction;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A rapid DNA minipreparation method was developed for rice and other plant species. This method uses an Eppendorf tube and 1-ml pipette tip to grind plant tissues, and requires only one transfer for DNA isolation. In a single day, one person can complete DNA isolation from more than 120 leaf samples. The yields of the DNA samples ranged from 2.3 to 5.2 μg from 25–50 mg fresh leaf tissue. DNA samples extracted using this method from rice were completely digested with five restriction enzymes (EcoR I, EcoR V, Hind III, Mse I and Pst I) and were successfully used for AFLP and other PCR applications.

引用

页码：53 / 57

页数：4

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