Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces

被引：10

作者：

Ijima H. ^{[1
]}

Murakami S. ^{[1
]}

Matsuo T. ^{[1
]}

Takei T. ^{[1
]}

Ono T. ^{[1
]}

Kawakami K. ^{[1
]}

机构：

[1] Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Higashi-ku, Fukuoka 812-8581

来源：

Journal of Artificial Organs | 2005年 / 8卷 / 2期

关键词：

Albumin secretion; Ammonia metabolism; Bone marrow cells; Co-culture; Hepatocyte;

D O I：

10.1007/s10047-005-0289-9

中图分类号：

学科分类号：

摘要：

Primary rat hepatocytes were co-cultured with bone marrow cells (BMCs) in a tissue culture-treated culture well (TCW) or a collagen-coated culture well (CCW). Although a medium containing serum was used, the co-cultured cells formed spheroids on the TCW within 2 days, and ammonia metabolism and albumin secretion activities were well maintained for 3 weeks. The co-cultured cells formed a monolayer on the CCW within 2 days, and liver functions were maintained for 3 weeks. The ammonia metabolism activities and albumin secretion activities of co-cultured cells on the CCWs were slightly inferior to those on the TCWs. This co-culture system of hepatocytes and BMCs is an appropriate culture system for the expression and maintenance of liver-specific functions in vitro, and is expected to be applicable to bioartificial liver systems, regenerative medicine, and liver function simulators. © The Japanese Society for Artificial Organs 2005.

引用

页码：104 / 109

页数：5

共 14 条

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