The vascular architecture of the chick chorioallantoic membrane: Sequential quantitative evaluation using corrosion casting

被引：29

作者：

Dimitropoulou C. ^{[1
,2
]}

Malkusch W. ^{[3
]}

Fait E. ^{[1
]}

Maragoudakis M.E. ^{[2
]}

Konerding M.A. ^{[1
,4
]}

机构：

[1] Department of Anatomy, University of Mainz

[2] Department of Pharmacology, Medical School, University of Patras

[3] Carl Zeiss Vision GmbH

[4] Anatomisches Institut, Makroskopischer Bereich, Johannes Gutenberg-Univ. Mainz

来源：

Angiogenesis | 1998年 / 2卷 / 3期

关键词：

Angiogenesis; Antiangiogenesis; Chorioallantoic membrane; Corrosion casting; Microvasculature; Network formation; Scanning electron microscopy; Vascular pattern;

D O I：

10.1023/A:1009210918738

中图分类号：

学科分类号：

摘要：

Microvascular corrosion casting was used for evaluating qualitatively and quantitatively angiogenesis in the chick chorioallantoic membrane (CAM). Series of CAMs from day 8 to 18 were examined. The density of plexus capillaries increases rapidly until day 10 and then remains constant. The vessels connected to the plexus (first order vessels) increase in number and length between days 10 and 12. The vessels initiating from first-order vessels (second order) also increase in number but remain nearly the same in lengths and diameters. Compared to previous studies using stereomicroscopy, significant differences in vessel numbers and lengths exist, which can be explained by the low resolution and magnification resulting in too low vascular densities of the pre- and post-capillaries. The third-order vessels increase in number until day 12 when they reach a plateau, whereas higher-order vessels increase in number. These results give an insight into the vascular development of this organ, and provide the basis for assessing the targets and effects of angiogenic or antiagiogenic agents.

引用

页码：255 / 263

页数：8

共 33 条

[1] McCormick J.E., Nassauer J., Bielunas J., Leighton J., Anatomy of the chick chorioallantoic membrane relevant to its use as an substrate in bioassay system, Scan. Electron Microsc., 4, pp. 2023-2030, (1984)
[2] Akins R.E., Tuan R.S., Transepithelial calcium transport in the chick chorioallantoic membrane. II. Compartmentalization of calcium during uptake, J Cell Sci, 105, pp. 381-388, (1993)
[3] Metcalfe J., Dissonnette J.M., Bowles R.E., Et al., Hen's eggs with retarded gas exchanges, Resp Physiol, 30, pp. 97-101, (1979)
[4] Knighton D., Ausprunk D., Tapper D., Folkman J., Avascular and vascular phases of tumour growth in the chick embryo, Br J Cancer, 35, pp. 347-356, (1977)
[5] Folkman J., Tumor angiogenesis, Adv Cancer Res, 43, pp. 173-203, (1985)
[6] Ribatti D., Vacca A., Roncali L., Dammacco F., The chick embryo chorioallantoic membrane as a model for in vivo research on angiogenesis, Int J Dev Biol, 40, pp. 1189-1197, (1996)
[7] Ribatti D., Bertossi M., Nico B., Et al., Role of basic fibroblast growth factor in the formation of the capillary plexus in the chick embryo chorioallantoic menbrane. An in situ hybridization, immunohistochemical and ultrastructural study, J Submicrosc Cytol Pathol, 30, pp. 127-136, (1998)
[8] DeFouw D.D., Rizzo V.J., Steinfeld R., Feinberg R.N., Mapping of the microcirculation in the chick chorioallantoic membrane during normal angiogenesis, Microvasc Res, 38, pp. 136-147, (1989)
[9] Christofferson R.H., Nilsson B.O., Microvascular corrosion casting in angiogenesis research, Scanning Electron Microscopy of Vascular Casts: Methods and Applications, pp. 27-37, (1992)
[10] Miodonski A.J., Hodde K.C., Bakker C., Rasterelektronenmikroskopie von Plastik-Korrosions-Präparaten

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