The effects of anther culture and plant genetic background on Agrobacterium rhizogenes-mediated transformation of commercial cultivars and derived doubled-haploid Brassica oleracea

被引：13

作者：

Cogan N. ^{[1
]}

Harvey E. ^{[1
]}

Robinson H. ^{[1
]}

Lynn J. ^{[1
]}

Pink D. ^{[1
]}

Newbury H. ^{[2
]}

Puddephat I. ^{[1
]}

机构：

[1] Horticulture Research International, Wellesbourne, Warwick

[2] School of Biological Sciences, University of Birmingham, Birmingham

来源：

Plant Cell Reports | 2001年 / 20卷 / 8期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Agrobacterium rhizogenes; Brassica oleracea; Double haploid; Genetic analysis; Transformation;

D O I：

10.1007/s00299-001-0395-y

中图分类号：

学科分类号：

摘要：

The production of transgenic roots was scored for eight Brassica oleracea cultivars from broccoli, cabbage, cauliflower and kale following inoculation with an Agrobacterium rhizogenes cell line carrying a binary plasmid bearing the green fluorescence protein (gfp) gene in the T-DNA. Significant differences in the numbers of explants producing transgenic roots were observed between cultivars, ranging from 1.4% for Marathon F1 to 57.8% for the Green Duke Fl. Three F1 cultivars were subjected to anther culture, and doubled-haploid (DH) lines were used for transformation. The DH lines produced showed considerable variation for transgenic root production with some lines showing increased efficiency compared to the parental F1 cultivar. Grouping of the DH lines into response classes with respect to transgenic root production allowed the development of potential genetic models to explain the variation in performance released from each F1 cultivar. No apparent segregation distortion for transgenic root production was observed in the DH lines following anther culture.

引用

页码：755 / 762

页数：7

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