Quantitative PCR tissue expression profiling of the human SGLT2 gene and related family members

被引：247

作者：

Chen J. ^{[1
]}

Williams S.

Ho S.

Loraine H.

Hagan D. ^{[2
]}

Whaley J.M. ^{[2
]}

Feder J.N. ^{[1
]}

机构：

[1] Applied Biotechnology and the Department of Applied Genomics, Bristol-Myers Squibb Company, Pennington, NJ 08534

[2] Department of Metabolic Diseases, Bristol-Myers Squibb R and D, Princeton, NJ

来源：

Diabetes Therapy | 2010年 / 1卷 / 2期

关键词：

quantitative PCR; SGLT2; sodiumglucose cotransporter protein; tissue expression; type; 2; diabetes;

D O I：

10.1007/s13300-010-0006-4

中图分类号：

学科分类号：

摘要：

SGLT2 (for "Sodium GLucose coTransporter" protein 2) is the major protein responsible for glucose reabsorption in the kidney and its inhibition has been the focus of drug discovery efforts to treat type 2 diabetes. In order to better clarify the human tissue distribution of expression of SGLT2 and related members of this cotransporter class, we performed TaqMan TM (Applied Biosystems, Foster City, CA, USA) quantitative polymerase chain reaction (PCR) analysis of SGLT2 and other sodium/glucose transporter genes on RNAs from 72 normal tissues from three different individuals. We consistently observe that SGLT2 is highly kidney specific while SGLT5 is highly kidney abundant; SGLT1, sodium-dependent amino acid transporter (SAAT1), and SGLT4 are highly abundant in small intestine and skeletal muscle; SGLT6 is expressed in the central nervous system; and sodium myoinositol cotransporter is ubiquitously expressed across all human tissues. © 2010 Springer Healthcare.

引用

页码：57 / 92

页数：35

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