Curcumin Suppresses the Production of Pro-inflammatory Cytokine Interleukin-18 in Lipopolysaccharide Stimulated Murine Macrophage-Like Cells

被引：43

作者：

Yadav R. ^{[1
]}

Jee B. ^{[1
,2
]}

Awasthi S.K. ^{[1
]}

机构：

[1] Institute of Life Sciences, Chhatrapati Shahu Ji Maharaj University, Kanpur

[2] Department of Microbiology and Molecular Biology, National JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Agra

来源：

Indian Journal of Clinical Biochemistry | 2015年 / 30卷 / 1期

关键词：

Curcumin; IL-18; Murine macrophage-like cells; Necrosis;

D O I：

10.1007/s12291-014-0452-2

中图分类号：

学科分类号：

摘要：

Curcumin is a major bioactive compound of turmeric that exerts its anti-inflammatory effects by suppressing the many pro-inflammatory cytokines and chemokines in a number of cell types and pathologic conditions. Interleukin-18 (IL-18) is a novel pro-inflammatory cytokine which plays an important role not only in generating Th1 responses but also in inducing severe inflammatory reactions. As curcumin induced inhibition of IL-18 production in keratinocytes and mice is well known, effect of curcumin on IL-18 release in macrophages remains unknown. Hence, this present study has been designed to evaluate the effect of curcumin on IL-18 production and necrotic cell death in murine macrophages-like cells treated with or without lipopolysaccharide (LPS). The IL-18 secretion in cell culture supernatants was assayed by enzyme-linked immunosorbent assay and cytotoxicity was determined by lactate dehydrogenase release assay. Our results demonstrate that curcumin significantly inhibited the production of pro-inflammatory cytokine IL-18 in E.coli LPS stimulated murine macrophage-like cells RAW264.7 in a concentration-dependent manner. Interestingly, curcumin had no cytotoxic effect on murine macrophage-like cells. Our findings suggest that curcumin may be used as a potential therapeutic agent for the treatment of inflammatory diseases. © 2014, Association of Clinical Biochemists of India.

引用

页码：109 / 112

页数：3

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