A cDNA Encoding Polygalacturonase-inhibiting Protein Induced in Citrus Leaves by Polygalacturonase of Alternaria citri

被引:27
作者
Yukie GOTOH
Sarunya NALUMPANG
Atsunori ISSHIKI
Takami UTSUMI
Kenji GOMI
Hiroyuki YAMAMOTO
Kazuya AKIMITSU
机构
[1] Laboratory of Plant Pathology,
[2] Department of Life Sciences,undefined
[3] Faculty of Agriculture,undefined
[4] Kagawa University,undefined
[5] Miki,undefined
[6] Kagawa 761–0795,undefined
[7] Japan,undefined
[8] United Graduate School of Agriculture,undefined
[9] Kagawa University,undefined
[10] Miki,undefined
[11] Kagawa 761–0795,undefined
[12] Japan,undefined
关键词
Key words : Alternaria alternata, Alternaria citri, citrus, polygalacturonase-inhibiting protein, polygalacturonase, rough lemon.;
D O I
10.1007/PL00013054
中图分类号
学科分类号
摘要
A cDNA encoding the polygalacturonase-inhibiting protein gene (RlemPGIPl) was identified from rough lemon (Citrus jambhiri Lush.). The deduced amino acid sequence is 46-75% identical to other plant PGIPs. The transcripts of the PGIP gene were weakly but constitutively expressed in leaves and increased in leaves within 30 min after wounding or inoculation with nonpathogenic Alternaria alternata or A. citri. However, expression of the PGIP and chalcone synthase genes did not increase after inoculation with an endopolygalacturonase (endoPG)-disrupted mutant of A. citri. These results indicate that endoPG may act as an elicitor for induction of these genes in citrus, most likely caused by PGIP interactions.
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页码:57 / 61
页数:4
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