In vivo involvement of polymorphonuclear neutrophils in Leishmania infantum infection

被引：44

作者：

Rousseau D. ^{[1
]}

Demartino S. ^{[1
]}

Ferrua B. ^{[1
]}

Michiels J.F. ^{[2
]}

Anjuère F. ^{[1
]}

Fragaki K. ^{[1
]}

Le Fichoux Y. ^{[1
,2
]}

Kubar J. ^{[1
]}

机构：

[1] INSERM U364, IFR50, Faculté de Médecine

[2] Laboratoire de Parasitologie, Hôpital de l'Archet

来源：

BMC Microbiology | / 1卷 / 1期

关键词：

Visceral Leishmaniasis; Parasite Load; Parasite Growth; Parasite Multiplication; Leishmania Infection;

D O I：

10.1186/1471-2180-1-17

中图分类号：

学科分类号：

摘要：

Background: The role of lymphocytes in the specific defence against L. infantum has been well established, but the part played by polynuclear neutrophil (PN) cells in controlling visceral leishmaniasis was much less studied. In this report we examine in vivo the participation of PN in early and late phases of infection by L. infantum. Results: Promastigote phagocytosis and killing occurs very early after infection, as demonstrated by electron microscopy analyses which show in BALB/c mouse spleen, but not in liver, numerous PN harbouring ultrastructurally degraded parasites. It is shown, using mAb RB6-8C5 directed against mature mouse granulocytes, that in chronically infected mice, long-term PN depletion did not enhance parasite counts neither in liver nor in spleen, indicating that these cells are not involved in the late phase of L. infantum infection. In acute stage of infection, in mouse liver, where L. infantum load is initially larger than that in spleen but resolves spontaneously, there was no significant effect of neutrophils depletion. By contrast, early in infection the neutrophil cells crucially contributed to parasite killing in spleen, since PN depletion, performed before and up to 7 days after the parasite inoculation, resulted in a ten-fold increase of parasite burden. Conclusions: Taken together these data show that neutrophil cells contribute to the early control of the parasite growth in spleen but not in liver and that these cells have no significant effect late in infection in either of these target organs.

引用

页码：1 / 7

页数：6

共 37 条

[1]

Murray H.W., Masur H., Keithl J.S., Cell-mediated immune response in experimental visceral leishmaniasis. Correlation between resistance to Leishmania donovani and lyphokine-generating capacity, J Immunol, 129, pp. 344-349, (1982)

[2]

Murray H.W., Gamma interferon, cytokine-induced macrophage activation, and antimicrobial host defense. In vitro, in animal models, and in humans, Diagn Microbiol Infect Dis, 13, pp. 411-421, (1990)

[3]

Murray H.W., Squires K.E., Miralles C.D., Stoeckle M.Y., Granger A.M., Granelli-Piperno A., Bogdan C., Acquired resistance and granuloma formation in experimental visceral leishmaniasis. Differential T cell and lymphokine roles in initial versus established immunity, J Immunol, 148, pp. 1858-1863, (1992)

[4]

Buffet P.A., Sulahian A., Gari Y.J.F., Nassar N., Derouin F., Culture microtitration: A sensitive method for quantifying Leishmania infantum in tissues if infected mice, Antimicrob Agents Chemother, 39, pp. 2167-2168, (1995)

[5]

Leclercq V., Lebastard M., Belkaid Y., Louis J., Milon G., The outcome of the parasitic process initiated by Leishmania infantum in laboratory mice, J Immunol, 157, pp. 4537-4543, (1996)

[6]

Wilson M.E., Sandor M., Blum A.M., Young B.M., Metwali A., Elliott D., Lynch R.G., Weinstock J.V., Local suppression of IFNγ in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi, J Immunol, 156, pp. 2231-2238, (1996)

[7]

Cotterell S.E.J., Engwerda C.R., Kaye P.M., Leishmania donovani infection initiates T-cell independent chemokine responses, which are subsequently amplified in a T-cell dependent manner, Eur J Immunol, 29, pp. 203-214, (1999)

[8]

Rousseau D., Le Fichoux Y., Stien X., Suffia I., Ferrua B., Kubar J., Progression of visceral leishmaniasis due to Leishmania infantum in BALB/c mice is markedly slowed by prior infection with Trichinella spiralis, Infect Immun, 65, pp. 4978-4983, (1997)

[9]

Le Fichoux Y., Rousseau D., Ferrua B., Ruette S., Lelievre A., Grousson D., Kubar J., Short- and long-term efficacy of hexadecylphosphocholine against established Leishmania infantum infection in BALB/c mice, Antimicrob Agents Chemother, 42, pp. 654-658, (1998)

[10]

Rousseau D., Suffia I., Ferrua B., Philip P., Le Fichoux Y., Kubar J., Prolonged administration of dexamethasone induces limited reactivation of visceral leishmaniosis in chronically infected BALB/c mice, Eur Cytokine Netw, 9, pp. 655-661, (1998)

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