Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells

被引：24

作者：

Ying Chen

Yiqin Wang

Yongru Sun

Liming Zhang

Wenbin Li

机构：

[1] Group 603,

[2] Institute of Genetics,undefined

[3] Chinese Academy of Sciences,undefined

[4] Beijing 100101,undefined

[5] P.R. China,undefined

[6] Group 603,undefined

[7] Institute of Genetics,undefined

[8] Chinese Academy of Sciences,undefined

[9] Beijing 100101,undefined

[10] P.R. China,undefined

[11] Present address: Laboratory of Phytopathology and Plant Protection,undefined

[12] Katholieke Universiteit Leuven,undefined

[13] Willem de Croylaan 42,undefined

[14] 3001 Heverlee,undefined

[15] Belgium,undefined

来源：

Current Genetics | 2001年 / 39卷

关键词：

Algae Chlorella ellipsoidea Highly efficient transformation Rabbit NP-1 gene;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A highly efficient system was developed for the expression of foreign genes in Chlorella ellipsoidea cells. The effect of five promoters on the expression efficiency of β-glucuronidase (GUS) gene was evaluated by transient expression of the UidA gene. Among these promoters, Ubiquitin-Ω was found to be the most efficient and was selected to drive the expression of foreign genes in Chlorella cells. A gene encoding the mature rabbit neutrophil peptide-1 (NP-1) was introduced into the cells. Integration of the gene for NP-1 into the Chlorella genome was confirmed by PCR and Southern blot analysis. In vitro anti-microbial testes demonstrated the expression of biologically active NP-1 by the transgenic Chlorella cells.

引用

页码：365 / 370

页数：5