Monitoring expression profiles of Arabidopsis genes during cold acclimation and deacclimation using DNA microarrays

被引：131

作者：

Oono Y. ^{[1
,2
,3
]}

Seki M. ^{[1
,3
]}

Satou M. ^{[1
,7
]}

Iida K. ^{[1
]}

Akiyama K. ^{[1
,7
]}

Sakurai T. ^{[1
,7
]}

Fujita M. ^{[1
,5
]}

Yamaguchi-Shinozaki K. ^{[4
,5
,6
]}

Shinozaki K. ^{[4
,5
,6
]}

机构：

[1] Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, Tsurumi-ku, Yokohama 230-0045

[2] Graduate School of Life and Environmental Science, University of Tsukuba, Tsukuba, Ibaraki 305-0074

[3] Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, Tsukuba 305-0074

[4] Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki 305-8686

[5] CREST, Japan Science and Technology Corporation (JST)

[6] Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyou-ku, Tokyo 113-8657

[7] RIKEN Plant Science Center, Tsurumi-ku, Yokohama 230-0045

来源：

Functional & Integrative Genomics | 2006年 / 6卷 / 3期

基金：

日本科学技术振兴机构;

关键词：

Cold acclimation; Cold stress; Deacclimation; Recovery from cold stress;

D O I：

10.1007/s10142-005-0014-z

中图分类号：

学科分类号：

摘要：

A comparative analysis of gene expression profiles during cold acclimation and deacclimation is necessary to elucidate the molecular mechanisms of cold stress responses in higher plants. We analyzed gene expression profiles in the process of cold acclimation and deacclimation (recovery from cold stress) using two microarray systems, the 7K RAFL cDNA microarray and the Agilent 22K oligonucleotide array. By both microarray analyses, we identified 292 genes up-regulated and 320 genes down-regulated during deacclimation, and 445 cold up-regulated genes and 341 cold down-regulated genes during cold acclimation. Many genes up-regulated during deacclimation were found to be down-regulated during cold acclimation, and vice versa. The genes up-regulated during deacclimation were classified into (1) regulatory proteins involved in further regulation of signal transduction and gene expression and (2) functional proteins involved in the recovery process from cold-stress-induced damages and plant growth. We also applied expression profiling studies to identify the key genes involved in the biosynthesis of carbohydrates and amino acids that are known to play important roles in cold acclimation. We compared genes that are regulated during deacclimation with those regulated during rehydration after dehydration to discuss the similarity and difference of each recovery process. © Springer-Verlag 2006.

引用

页码：212 / 234

页数：22

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