Collateral sprouting mechanism after end-to-side nerve repair in the rat

被引：25

作者：

Yamauchi T. ^{[1
]}

Maeda M. ^{[1
]}

Tamai S. ^{[1
]}

Tamai M. ^{[1
]}

Yajima H. ^{[1
]}

Takakura Y. ^{[1
]}

Haga S. ^{[1
]}

Yamamoto H. ^{[1
]}

机构：

[1] Department of Orthopaedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara

来源：

Medical Electron Microscopy | 2000年 / 33卷 / 3期

关键词：

BDNF; Collateral sprouting; End-to-side neurorrhaphy; GAP-43; NT-3;

D O I：

10.1007/s007950000015

中图分类号：

学科分类号：

摘要：

The collateral sprouting mechanisms of axons from an uninjured donor nerve after end-to-side nerve repair was investigated in motor nerves of rats, with special reference to the neurotrophins related to nerve regeneration. In addition, growth cone formation at the tip of the regenerating nerve was examined. A transected medial gastrocnemius nerve (MGN) was transferred to the side of an intact lateral gastrocnemius nerve (LGN) using a Y-shaped silicone tube. At 3, 7, or 14 days later, the MGN with the LGN was transected and was stained immunohistologically. Expression of neurotrophin-3 (NT-3) and Trk C (receptor of NT-3) was most significantly observed 3 days postoperatively around the site of coaptation. Brain-derived neurotrophic factor (BDNF) and Trk B (receptor of BDNF) was weakly detected at the coaptation site 3 days after-operation. Growth-associated protein 43 (GAP-43), which is a marker of growth cone formation, was observed at the site of coaptation in the LGN 7 days postoperatively and in the MGN at the site of coaptation at 14 days. We concluded that motor nerve regeneration due to collateral sprouting of axons after end-to-side nerve repair is possible. We thus demonstrated the involvement of at least one neurotrophin, NT-3, in the process of collateral sprouting of motor nerves.

引用

页码：151 / 156

页数：5

共 12 条

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