Adventitious shoot formation and plant regeneration from Pinus pinaster sol. ex aiton

被引：17

作者：

Calixto F. ^{[1
]}

Pais M.S. ^{[1
]}

机构：

[1] Centro de Biotecnologia Vegetal, Faculdade de Cie. de Lisboa, Edifício C2, Campo Grande

来源：

In Vitro Cellular & Developmental Biology - Plant | 1997年 / 33卷 / 2期

关键词：

Cotyledon; Microprogation; Organogenesis; Pinus pinaster; Rhizogenesis;

D O I：

10.1007/s11627-997-0008-8

中图分类号：

学科分类号：

摘要：

Pinus pinaster plants were regenerated from cotyledons excised from in vitro germinated seeds and axenically cultured on induction medium (GMD). 6-Benzyladenine (2.2 μM) induced the highest frequency of direct bud formation from cotyledons. An average of 13.1 ± 2.1 elongated shoots per cotyledon was obtained. Germination time influenced shoot induction, and the organogenic potential decreased with explant age. Cotyledons remained for 21 d on induction medium, and in order to promote adventitious shoot elongation, they were transferred to Gupta and Durzan's DCR medium without growth regulators, containing 0.5% (wt/vol) activated charcoal and 3% (wt/vol) sucrose. Rooting was achieved by application of an indole-3-butyric acid, (396.6 μM) pulse (24 h at 4 °C), followed by transfer to a sterile mixture of peat plus perlite (1:1 vol/vol). Ninety-eight to 100% of the regenerated plants were successfully acclimatized. All plants have survived after transfer to the field.

引用

页码：119 / 124

页数：5

共 36 条

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