GM1 stabilizes expression of NMDA receptor subunit 1 in the ischemic hemisphere of MCAo/reperfusion rat

被引:16
作者
Liu Jian-ren
Ding Mei-ping
Wei Er-qing
Luo Jian-hong
Song Ying
Huang Jian-zheng
Ge Qiu-fu
Hu Hua
Zhu Li-jun
机构
[1] Zhejiang University,Department of Neurology, Second Affiliated Hospital, School of Medicine
[2] Zhejiang University,Department of Neurosurgery, Second Affiliated Hospital, School of Medicine
[3] Zhejiang University,Department of Pharmacology, School of Medicine
[4] Zhejiang University,Department of Neurobiology, School of Medicine
来源
Journal of Zhejiang University Science B | 2005年 / 6卷 / 4期
关键词
G(M1) ganglioside; Middle cerebral artery occlusion; Reperfusion; N-methyl-D-aspartate receptors; Rats; A; R741;
D O I
10.1007/BF02842461
中图分类号
学科分类号
摘要
Objective: To determine the protective effect of monosialoganglionside (GM1) and evaluate the influence of GMI on expression of N-methyl-D-aspartate receptor subunit 1 (NMDAR1) in Sprague-Dawley (SD) rats with focal cerebral ischemia-reperfusion (I/R). Methods: Left middle cerebral artery (MCA) was occluded by an intraluminal suture for 1 h and the brain was reperfused for 72 h in SD rats when infarct volume was measured. GM1 (10 mg/kg) was givenip (intraperitoneally) at 5 min (group A), 1 h (group B) and 2h (group C) after MCA occlusion (MCAo). Expression of NMDAR1 was detected by Western blot at various time after reperfusion (4 h, 6 h, 24 h, 48 h and 72 h) in ischemic hemispheres of the rats with or without GM1 administered. Results: (1) Adjusted relative infarct volumes of groups A and B were significantly smaller than that of group C and the control group (P<0.01, andP<0.05, respectively). (2) Expression level of NMDAR1 was temporally high at 6 h after reperfusion, and dipped below the normal level at 72 h after reperfusion. GM1 at 5 min after MCAo significantly suppressed the expression of NMDAR1 at 6 h after reperfusion (P<0.05 vs the control). At 72 h after reperfusion, the NMDAR1 expression level of rats treated with GM1 administered (at 5 min or 2 h after MCAo) was significantly higher than that of the control (P<0.05). Conclusion: GM1 can time-dependently reduce infarct volume in rats with focal cerebral I/R partly through stabilizing the expression of NMDAR1.
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页码:254 / 258
页数:4
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