The structure of Pariacoto virus reveals a dodecahedral cage of duplex RNA

被引：151

作者：

Tang L. ^{[1
]}

Johnson K.N. ^{[2
]}

Ball L.A. ^{[2
]}

Lin T. ^{[1
]}

Yeager M. ^{[1
,3
,4
]}

Johnson J.E. ^{[1
]}

机构：

[1] Department of Molecular Biology, Scripps Research Institute, San Diego, CA 92037

[2] Department of Microbiology, University of Alabama at Birmingham, BBRB 373/17, S. Birmingham, AL 35294

[3] Department of Cell Biology, Scripps Research Institute, San Diego, CA 92037

[4] Division of Cardiovascular Diseases, Scripps Clinic, San Diego, CA 92037

来源：

Nature Structural Biology | 2001年 / 8卷 / 1期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/83089

中图分类号：

学科分类号：

摘要：

The 3.0 Å resolution crystal structure of Pariacoto virus (PaV) reveals extensive interactions between portions of the viral RNA genome and the icosahedral capsid. Under the protein shell of the T = 3 quasi equivalent capsid lies a dodecahedral cage composed of RNA duplex that accounts for ∼35% of the single-stranded RNA genome. The highly basic N-terminal regions (residues 7-54) of the subunits, forming pentamers (A subunits) are clearly visible in the density map and make numerous interactions with the RNA cage. The C-terminal segments (residues 394-401) of the A subunits lie in channels near the quasi three-fold axes. Electron cryo-microscopy and image reconstruction of PaV particles clearly show the dodecahedral RNA cage.

引用

页码：77 / 83

页数：6

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