Vitamin C blocks TNF-α-induced NF-kB activation and ICAM-1 expression in human neuroblastoma cells

被引：2

作者：

Eun -Wha Son

Sung -Ji Mo

Dong -Kwon Rhee

Suhkneung Pyo

机构：

[1] Samcheok National University,Dept. of Pharmacognosy Material Development

[2] College of Pharmacy Sungkyunkwan University,Division of Immunopharmacology

来源：

Archives of Pharmacal Research | 2004年 / 27卷

关键词：

Vitamin C; ICAM-1; TNF-α; NF-; B;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Interactions of the cell adhesion molecules are known to play important roles in mediating inflammation. The proinflammatory cytokine, tumor necrosis factor-α (TNF-α), activates the NF-kB signaling pathway, which induces the expression of various genes, such as intercellular adhesion molecule-1 (ICAM-1). In this study, the effect of vitamin C on the ICAM-1 expression induced by TNF-α in a human neuroblastoma cell line, SK-N-SH was investigated. Treatment with vitamin C resulted in the downregulation of the TNF-α-induced surface expression and ICAM-1 mRNA levels in a concentration-dependent manner. Moreover, a gel shift analysis indicated that vitamin C dose-dependently inhibited the NF-kB activation and lkBα degradation induced by TNF-α. Taken together, these results suggest that vitamin C downregulates TNF-α-induced ICAM-1 expression via the inhibition of NF-kB activation.

引用

页码：1073 / 1079

页数：6

共 133 条

[1]

Albelda S. M.(1994)Adhesion molecules and inflammatory injury FASEB J. 8 504-512

[2]

Smith C. W.(1994)Function and activation of NF-kappa B in the immune system Annu. Rev. Immunol. 12 141-179

[3]

Ward P. A.(1991)Induction of ICAM-1 on human neural cells and mechanisms of neutrophil-mediated injury Am. J. Pathol. 139 1341-1350

[4]

Baeuerle P. A.(1992)Induction of VCAM-1 and ICAM-1 on human neural cells and mechanisms of mononuclear leukocyte adherence J. Immunol. 148 2717-2723

[5]

Henkel T.(2000)Secondary prevention with antioxidants of cardiovascular disease in end-stage renal disease (SPACE): randomised placebo controlled trial Lancet 356 1213-1218

[6]

Birdsall H. H.(2000)Vitamin C inhibits NF-kB activation by TNF via the activation of p38 mitogen-activated protein kinase J. Immunol. 165 7180-7188

[7]

Birdsall H. H.(2001)Tumor necrosis factor a-induced activation of downstream Cell Signal. 13 543-553

[8]

Lane C.(1987) site of the promoter mediates epithelial ICAM-1 expression and monocyte adhesion: involvement of PKCα, tyrosine kinase, and IKK2, but not MAPKs pathway Anal. Biochem. 162 156-159

[9]

Ramser M. N.(1993)Single-step method of RNA isolation by guanidinium thiocyanate-phenol-chloroform extraction J. Pathol. 171 223-229

[10]

Anderson D. C.(1995)The expression of the adhesion molecules ICAM-1, VCAM-1, PECAM, and E- selectin in human atherosclerosis Immunity 3 263-272

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