Amino Acid Changes in Pepper mild mottle virus Coat Protein That Affect L3 Gene-mediated Resistance in Pepper

被引：27

作者：

Hiroyuki HAMADA

Shigeharu TAKEUCHI

Akinori KIBA

Shinya TSUDA

Yasufumi HIKICHI

Tetsuro OKUNO

机构：

[1] Laboratory of Plant Pathology and Biotechnology,

[2] Faculty of Agriculture,undefined

[3] Kochi University,undefined

[4] Nankoku 782-8502,undefined

[5] Japan,undefined

[6] Laboratory of Plant Pathology,undefined

[7] Kochi Agricultural Research Center,undefined

[8] Nankoku 783-0023,undefined

[9] Japan,undefined

[10] National Agriculture Research Center,undefined

[11] Tsukuba 305-8666,undefined

[12] Japan,undefined

[13] Laboratory of Plant Pathology,undefined

[14] Graduate School of Agriculture,undefined

[15] Kyoto University,undefined

[16] Kyoto 606-8502,undefined

[17] Japan,undefined

来源：

Journal of General Plant Pathology | 2002年 / 68卷 / 2期

关键词：

Key words : Pepper mild mottle virus, Tobamovirus, HR, L gene, virus resistance, Capsicum chinense.;

D O I：

10.1007/PL00013069

中图分类号：

学科分类号：

摘要：

Several field isolates of Pepper mild mottle virus (PMMoV) were obtained from Capsicum annuum (L3/ + ) that exhibited systemic mosaic or systemic necrosis. The deduced amino acid sequences of the coat protein (CP) of these isolates differed from those of previously reported PMMoV strains that are able to overcome L3gene-mediated resistance. CP mutants having these amino acid changes were constructed using cDNA clones pTPWl and pTPC4350. These clones produce in vitro transcripts TPW1, which induces a resistance response resulting in local infection, and TPC4350, which causes no response on inoculated leaves resulting in systemic infection in the Capsicum plants carrying the L3gene (L3/L3). These CP mutants were tested for infection and symptomatology in Capsicum spp. plants (L3/L3 and L3/ + ). An A/S (from serine to alanine) change at position 81 in the CP of TPW1 conferred a resistance-breaking activity in the L3/L3plants, whereas an S/A change at position 7 in the CP of either TPW1 or TC4350 conferred resistance-inducing activities. These results suggest that amino acid changes of A/S at position 81 and S/A at position 7 in CP had inverse effects on the L3gene-mediated resistance. These and other amino acid changes in PMMoV CP are also discussed in relation to the dosage effects of the L3gene on virus resistance.

引用

页码：155 / 162

页数：7