Construction and analysis of a substracted cDNA library ofBetula platyphylla female inflorescence

被引：7

作者：

Wei Ji-cheng

Yang Chuan-ping

Wang Chao

Jiang Jing

机构：

[1] Northeast Forestry University,Forestry College

[2] Mudanjiang Teachers College,undefined

来源：

Journal of Forestry Research | 2005年 / 16卷 / 2期

关键词：

Subtracted cDNA library; SMART; S792.153; A;

D O I：

10.1007/BF02857898

中图分类号：

学科分类号：

摘要：

Female inflorescence ofBetula platyphylla was sampled at an interval of each two days to analyze the background of gene expression in floral phase. On the basis of SMART strategy, the driver cDNA was obtained from total RNA of the last sample and the tester cDNA was from that of the others by RT-PCR which were subsequently used to construct a subtracted cDNA library. The result of the ESTs (expression sequence tags) blastX showed that the genes in the subtracted cDNA library could be mainly clustered into 5 groups related to metabolism, transportation and signal transduction, cell cycle, stress response, and regulation. The relationship between gene expression and development was also discussed.

引用

页码：97 / 100

页数：3

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