Improvements to parallel plate flow chambers to reduce reagent and cellular requirements

被引：38

作者：

Brown D.C. ^{[1
]}

Larson R.S. ^{[1
]}

机构：

[1] Department of Pathology, Univ. of New Mexico Sch. of Medicine, Albuquerque, NM

来源：

BMC Immunology | / 2卷 / 1期

关键词：

Flow Chamber; Dead Volume; Rolling Velocity; Chamber Design; Small Molecule Antagonist;

D O I：

10.1186/1471-2172-2-9

中图分类号：

学科分类号：

摘要：

Background: The parallel plate flow chamber has become a mainstay for examination of leukocytes under physiologic flow conditions. Several design modifications have occurred over the years, yet a comparison of these different designs has not been performed. In addition, the reagent requirements of many designs prohibit the study of rare leukocyte populations and require large amounts of reagents. Results: In this study, we evaluate modifications to a newer parallel plate flow chamber design in comparison to the original parallel plate flow chamber described by Lawrence et al. We show that modifications in the chamber size, internal tubing diameters, injection valves, and a recirculation design may dramatically reduce the cellular and reagent requirements without altering measurements. Conclusions: These modifications are simple and easily implemented so that study of rare leukocyte subsets using scarce or expensive reagents can occur. © 2001 Brown and Larson; licensee BioMed Central Ltd.

引用

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