The chemical-in-plug bacterial chemotaxis assay is prone to false positive responses

被引：16

作者：

Li J. ^{[2
]}

Go A.C. ^{[1
]}

Ward M.J. ^{[2
,3
]}

Ottemann K.M. ^{[1
,3
]}

机构：

[1] Department of Microbiology and Environmental Toxicology, UC Santa Cruz, Santa Cruz

[2] Department of Geography and Environmental Engineering, Johns Hopkins University, Baltimore, MD 21218

[3] Department of Earth Sciences, Los Angeles, CA 90089

来源：

BMC Research Notes | / 3卷 / 1期

关键词：

TMAO; Chemotactic Response; Chemotaxis Assay; Bacto Agar; Bacterial Chemotaxis;

D O I：

10.1186/1756-0500-3-77

中图分类号：

学科分类号：

摘要：

Background. Chemical-in-plug assays are commonly used to study bacterial chemotaxis, sometimes in the absence of stringent controls. Results. We report that non-chemotactic and non-motile mutants in two distinct bacterial species (Shewanella oneidensis and Helicobacter pylori) show apparent zones of accumulation or clearing around test plugs containing potential attractants or repellents, respectively. Conclusions. Our results suggest that the chemical-in-plug assay should be used with caution, that non-motile or non-chemotactic mutants should be employed as controls, and that results should be confirmed with other types of assays. © 2010 Ward et al; licensee BioMed Central Ltd.

引用

共 26 条

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