Expression of three distinct families of calcium-activated chloride channel genes in the mouse dorsal root ganglion

被引：28

作者：

Al-Jumaily M. ^{[1
]}

Kozlenkov A. ^{[1
]}

Mechaly I. ^{[1
,2
]}

Fichard A. ^{[1
,2
]}

Matha V. ^{[1
]}

Scamps F. ^{[1
]}

Valmier J. ^{[1
,2
]}

Carroll P. ^{[1
]}

机构：

[1] French National Institute for Health and Medical Research, U.583, Montpellier

[2] University Montpellier II, Montpellier

来源：

Neuroscience Bulletin | 2007年 / 23卷 / 5期

关键词：

Bestrophin; Chloride channel; Chloride channel calcium activated; Dorsal root ganglion; Tweety;

D O I：

10.1007/s12264-007-0044-8

中图分类号：

学科分类号：

摘要：

Objective: A calcium-activated chloride current (ICl(Ca)) has been observed in medium-sized sensory neurons of the dorsal root ganglion (DRG). Axotomy of the sciatic nerve induces a similar current in the majority of medium and large diameter neurons. Our aim is to identify the molecule(s) underlying this current. Methods: Using conventional and quantitative RT-PCR, we examined the expression in DRG of members of three families of genes, which have been shown to have ICl(Ca) current inducing properties. Results: We showed the detection of transcripts representing several members of these families, i.e. chloride channel calciumactivated (CLCA), Bestrophin and Tweety gene families in adult DRG, in the normal state and 3 d after sciatic nerve section, a model for peripheral nerve injury. Conclusion: Our analysis revealed that that mBest1 and Tweety2 appear as the best candidates to play a role in the injury-induced ICl(Ca) in DRG neurons.

引用

页码：293 / 299

页数：6

共 27 条

[1]

Andre S., Boukhaddaoui H., Campo B., Al-Jumaily M., Mayeux V., Greuet D., Et al., Axotomy-induced expression of calcium-activated chloride current in subpopulations of mouse dorsal root ganglion neurons, J Neurophysiol, 90, pp. 3764-3773, (2003)

[2]

Campbell H.D., Kamei M., Claudianos C., Woollatt E., Sutherland G.R., Suzuki Y., Et al., Human and mouse homologues of the Drosophila melanogaster tweety (tty) gene: A novel gene family encoding predicted transmembrane proteins, Genomics, 68, pp. 89-92, (2000)

[3]

Chien L.T., Zhang Z.R., Hartzell H.C., Single Cl- channels activated by Ca2+ in Drosophila S2 cells are mediated by bestrophins, J Gen Physiol, 128, pp. 247-259, (2006)

[4]

Frings S., Reuter D., Kleene S.J., Neuronal Ca2+ -activated Cl-channels - homing in on an elusive channel species, Prog Neurobiol, 60, pp. 247-289, (2000)

[5]

Fuller C.M., Ji H.L., Tousson A., Elble R.C., Pauli B.U., Benos D.J., Ca (2+)-activated Cl(-) channels: A newly emerging anion transport family, Pflugers Arch, 443, SUPPL. 1, (2001)

[6]

Gibson A., Lewis A.P., Affleck K., Aitken A.J., Meldrum E., Thompson N., hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels, J Biol Chem, 280, pp. 27205-27212, (2005)

[7]

Greenwood I.A., Leblanc N., Overlapping pharmacology of Ca2+-activated Cl- and K+ channels, Trends Pharmacol Sci, 28, pp. 1-5, (2007)

[8]

Gruber A.D., Elble R.C., Pauli B.U., Discovery and cloning of the CLCA gene family, Current topics in Membranes, 53, pp. 367-387, (2002)

[9]

Hartzell C., Putzier I., Arreola J., Calcium-activated chloride channels, Annu Rev Physiol, 67, pp. 719-758, (2005)

[10]

Thompson J.D., Higgins D.G., Gibson T.J., CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids Res, 22, pp. 4673-4680, (1994)

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