Effect of interlukin-1β on proliferation of gastric epithelial cells in culture

被引：35

作者：

Beales I.L.P. ^{[1
]}

机构：

[1] Department of Cell Biology, Sch. of Med. Health Policy/Practice, University of East Anglia

来源：

BMC Gastroenterology | / 2卷 / 1期

关键词：

Pylorus Infection; Thymidine Incorporation; Gastric Epithelial Cell; Growth Stimulatory Action; RGM1 Cell;

D O I：

10.1186/1471-230X-2-7

中图分类号：

学科分类号：

摘要：

Background: Helicobacter pylori is the main risk factor for the development of non-cardia gastric cancer. Increased proliferation of the gastric mucosa is a feature of H. pylori infection. Mucosal interkeukin-1β production is increased in H. pylori infection and IL-1β genotypes associated with increased pro-inflammatory activity are risk factors for the development of gastric cancer. The effect of IL-1β on gastric epithelial cell proliferation has been examined in this study. Methods: AGS cells were cultured with IL-1β. DNA synthesis was assed by [3H]thymidine incorporation and total viable cell numbers by MTT assay. Results: IL-1β dose dependently increased DNA synthesis and cell numbers. The enhanced proliferation was blocked by interleukin-1 receptor antagonist. Addition of neutralising antibody to GM-CSF reduced IL-1β-stimulated proliferation by 31 ± 4 %. GM-CSF alone significantly stimulated proliferation. Addition or neutralisation of IL-8 had no effect on basal or IL-1β-stimulated proliferation. The tyrosine kinase inhibitor genistein completely blocked IL-1β -stimulated proliferation and inhibition of the extracellular signal related kinase pathway with PD 98059 inhibited IL-1β stimulated proliferation by 58 ± 5 %. Conclusions: IL-1β stimulates proliferation in gastric epithelial cells. Autocrine stimulation by GMCSF contributes to this proliferative response. Signalling via tyrosine kinase activity is essential to the mitogenic response to IL-1β. The extracellular signal related kinase pathway is involved in, but not essential to downstream signalling. IL-1β may contribute to the hyperproliferation seen in H. pylori- infected gastric mucosa, and be involved in the carcinogenic process. © 2002 Beales; licensee BioMed Central Ltd.

引用

页数：8

共 54 条

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