MicroRNA targets in Drosophila

被引：949

作者：

Anton J Enright

Bino John

Ulrike Gaul

Thomas Tuschl

Chris Sander

Debora S Marks

机构：

[1] Computational Biology Center, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, 10021, NY

[2] Laboratory of Developmental Neurogenetics, The Rockefeller University, 1230 York Avenue, New York, 10021, NY

[3] Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, 10021, NY

[4] Columbia Genome Center, Russ Berrie Pavilion, 1150 St. Nicholas Avenue, New York, 10032, NY

来源：

Genome Biology | / 5卷 / 1期

关键词：

Additional Data File; Ecdysone; miRNA Sequence; miRNA Target; Target Site;

D O I：

10.1186/gb-2003-5-1-r1

中图分类号：

学科分类号：

摘要：

Background: The recent discoveries of microRNA (miRNA) genes and characterization of the first few target genes regulated by miRNAs in Caenorhabditis elegans and Drosophila melanogaster have set the stage for elucidation of a novel network of regulatory control. We present a computational method for whole-genome prediction of miRNA target genes. The method is validated using known examples. For each miRNA, target genes are selected on the basis of three properties: sequence complementarity using a position-weighted local alignment algorithm, free energies of RNA-RNA duplexes, and conservation of target sites in related genomes. Application to the D. melanogaster, Drosophila pseudoobscura and Anopheles gambiae genomes identifies several hundred target genes potentially regulated by one or more known miRNAs. Results: These potential targets are rich in genes that are expressed at specific developmental stages and that are involved in cell fate specification, morphogenesis and the coordination of developmental processes, as well as genes that are active in the mature nervous system. High-ranking target genes are enriched in transcription factors two-fold and include genes already known to be under translational regulation. Our results reaffirm the thesis that miRNAs have an important role in establishing the complex spatial and temporal patterns of gene activity necessary for the orderly progression of development and suggest additional roles in the function of the mature organism. In addition the results point the way to directed experiments to determine miRNA functions. Conclusions: The emerging combinatorics of miRNA target sites in the 3' untranslated regions of messenger RNAs are reminiscent of transcriptional regulation in promoter regions of DNA, with both one-to-many and many-to-one relationships between regulator and target. Typically, more than one miRNA regulates one message, indicative of cooperative translational control. Conversely, one miRNA may have several target genes, reflecting target multiplicity. As a guide to focused experiments, we provide detailed online information about likely target genes and binding sites in their untranslated regions, organized by miRNA or by gene and ranked by likelihood of match. The target prediction algorithm is freely available and can be applied to whole genome sequences using identified miRNA sequences. © 2003, Enright et al.; licensee BioMed Central Ltd.

引用

共 87 条

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