High-throughput reverse genetics: RNAi screens in Caenorhabditis elegans

被引：37

作者：

Cornelia I Bargmann

机构：

[1] The University of California,Howard Hughes Medical Institute, Departments of Anatomy and of Biochemistry and Biophysics

来源：

Genome Biology | / 2卷 / 2期

关键词：

Mutant Phenotype; Differential Interference Contrast; RNAi Screen; Visible Phenotype; RNAi Phenotype;

D O I：

10.1186/gb-2001-2-2-reviews1005

中图分类号：

学科分类号：

摘要：

Two recent chromosome-wide screens for phenotypes caused by RNA-mediated interference (RNAi) in Caenorhabditis elegans have increased our understanding of essential genes in nematodes. These papers represent a major advance in functional genomics.

引用

共 39 条

[1] Fraser AG(2000)Functional genomic analysis of Nature 408 325-330
[2] Kamath RS(2000)chromosome I by systematic RNA interference. Nature 408 331-336
[3] Zipperlen P(1999)Functional genomic analysis of cell division in Trends Genet 15 358-363
[4] Martinez-Campos M(1998)using RNAi of genes on chromosome III. Science 282 430-431
[5] Sohrmann M(1998)RNA-triggered gene silencing. Nature 395 854-0002.10
[6] Ahringer J(2000)RNAi in Genome Biology 2 research0002.1-812
[7] Gonczy P(2000): soaking in the genome sequence. Science 290 809-220
[8] Echeverri G(2000)Specific interference by ingested dsRNA. Nat Genet 26 216-1622
[9] Oegema K(2000)Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Curr Biol 10 1619-undefined
[10] Coulson A(undefined)Genomic analysis of gene expression in undefined undefined undefined-undefined

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