Novel hand-held device for exhaled nitric oxide-analysis in research and clinical applications

被引：76

作者：

Hemmingsson T. ^{[1
,2
]}

Linnarsson D. ^{[1
]}

Gambert R. ^{[3
]}

机构：

[1] Sect. of Environmental Physiology, Dept. of Physiology and Pharmacology, Karolinska Institutet

[2] Aerocrine AB, SE-171 21 Solna

[3] International Technologies Dr. Gambert GmbH

来源：

Journal of Clinical Monitoring and Computing | 2004年 / 18卷 / 5-6期

关键词：

Asthma monitoring; Chemiluminescence; Electrochemical sensor; FENO;

D O I：

10.1007/s10877-005-1158-z

中图分类号：

学科分类号：

摘要：

Changes in expired nitric oxide (NO) occur in airway inflammation and have proved to be important in the monitoring of inflammatory disease processes such as asthma. We set out to develop a novel hand-held NO-analyzer with a performance comparable to the present more costly and complex chemiluminescence instruments. The new device is based on a specially designed electrochemical sensor, where we have developed a novel sampling and analysis technology, compensating for the relatively slow response properties of the electrochemical sensor technique. A Lowest Detection Limit in NO-analysis from reference gas tests of less than 3 ppb and a response time of 15 seconds together with an average precision in human breath measurements of 1.4 ppb were obtained. We also show an agreement with the existing 'gold standard' FENO measurement technique, within 0.5 ppb in a group of 19 subjects together with a high linearity and accuracy compared to reference gases. The new analyzer enables affordable monitoring of inflammatory airway diseases in research and routine clinical practice. © Springer Science + Business Media, Inc. 2005.

引用

页码：379 / 387

页数：8

共 13 条

[1] Gustafsson L.E., Leone A., Persson M.G., Wiklund N., Moncada S., Endogenous nitric oxide is present in the exhaled air of rabbits, guinea-pigs and humans, Biochem. Biophys. Res. Commun., 181, pp. 852-857, (1991)
[2] Alving K., Weitzberg E., Lundberg J.M., Increased amount of nitric oxide in exhaled air of asthmatics, Eur. Respir. J., 6, pp. 1368-1370, (1993)
[3] Smith A.D., Cowan J.O., Filsell S., McLachlan C., Monti-Sheehan G., Jackson P., Taylor R., Comparisons between exhaled nitric oxide measurements and conventional tests, Am. J. Respir. Crit. Care Med., 169, pp. 473-478, (2004)
[4] Payne D.N., Adcock I.M., Wilson N.M., Oates T., Scallan M., Bush A., Relationship between exhaled nitric oxide and mucosal eosinophilic inflammation in children with difficult asthma, after treatment with oral prednisolone, Am. J. Respir. Crit. Care Med., 164, pp. 1376-1381, (2001)
[5] Warke T.J., Fitch P.S., Brown V., Taylor R., Lyons J.D., Ennis M., Shields M.D., Exhaled nitric oxide correlates with airway eosinophils in childhood asthma, Thorax, 57, 5, pp. 383-387, (2002)
[6] Kharitonov S.A., Alving K., Barnes P.J., ERS Task Force Report: Exhaled and nasal nitric oxide measurements: Recommendations, Eur. Respir. J., 10, pp. 1683-1693, (1997)
[7] Recommendations for a standardized procedure for the on-line and off-line measurement of exhaled lower respiratory nitric oxide and nasal nitric oxide in adults and children, Am. J. Respir. Crit. Care Med., 160, pp. 2104-2117, (1999)
[8] Silkoff P.E., McClean P.A., Slutsky A.S., Suguru Wakita H.G., Chapman K.R., Szalai J.P., Zam N., Marked flow-dependence of exhaled nitric oxide using a new technique to exclude nasal nitric oxide, Am. J. Respir. Crit. Care Med., 155, pp. 260-267, (1997)
[9] Kharitonov S.A., Gonio F., Kelly C., Meah S., Barnes P.J., Reproducibility of exhaled nitric oxide measurements in healthy and asthmatic adults and children, Eur. Respir. J., 21, 3, pp. 433-438, (2003)
[10] Baraldi E., Azzolin N.M., Dario C., Carra S., Ongaro R., Biban P., Zacchello F., Effect of atmospheric nitric oxide (NO) on measurements of exhaled NO in asthmatic children, Pediatr. Pulmonol., 26, pp. 30-34, (1998)

← 1 2 →