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cDNA microarray analysis of gene expression in rice plants treated with probenazole, a chemical inducer of disease resistance
被引：18
作者：
Masaki Shimono
Junshi Yazaki
Keiko Nakamura
Naoki Kishimoto
Shoshi Kikuchi
Masataka Iwano
Kimiko Yamamoto
Katsumi Sakata
Takuji Sasaki
Masamichi Nishiguchi
机构：
[1] National Institute of Agrobiological Sciences,
[2] 2-1-2 Kan-nondai,undefined
[3] Tsukuba,undefined
[4] Ibaraki 305-8602,undefined
[5] Japan,undefined
[6] The Institute of the Society for Techno-innovation of Agriculture,undefined
[7] Forestry and Fisheries (STAFF),undefined
[8] Ibaraki,undefined
[9] Japan,undefined
[10] National Agricultural Research Center,undefined
[11] Ibaraki,undefined
[12] Japan,undefined
关键词：
Key words cDNA microarray;
Gene expression profile;
Probenazole;
Rice;
Rice blast fungus;
D O I：
10.1007/s10327-002-0010-2
中图分类号：
学科分类号：
摘要：
Probenazole (3-allyloxy-1,2-benzisothiazole-1,1-dioxide; PBZ) is a chemical inducer of disease resistance and has been used practically for protecting crops against pathogen infection. Using cDNA microarray, the expression of 1265 rice genes was monitored in rice plants after PBZ treatment. Ten cDNA clones exhibited more than threefold overexpression in the PBZ-treated plants compared to untreated controls. Some of the induced genes have identities with disease resistance- and pathogenesis-related genes. The enhanced gene expression was confirmed for 9 of the 10 genes using Northern blot or reverse transcription-polymerase chain reaction analysis. Expression of several clones was further investigated using RNA from rice plants infected with blast fungus. Under the conditions used, expression of three genes also showed an increased level of mRNA accumulation in fungus-infected plants. These results suggested that the genes enhanced during PBZ treatment were involved in the interactions between plants and pathogens.
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页码：76 / 82
页数：6
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