Cloning and expression of defibrase cDNA from the venom of Gloydius shedaoensis* *

被引：1

作者：

Qing Yang

Xiaoming Xu

Min Li

Xiaodong Yuan

Zhiguo Su

Jan-Christer Janson

Lijia An

机构：

[1] Dalian University of Technology,Bioengineering Institute

[2] Takara Biotech Dalian Co. Ltd.,State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy

[3] Chinese Academy of Sciences,Biosurface Science Center

[4] Biomedical Center,Bioengineering Institute

[5] Dalian University of Technology,undefined

来源：

Biotechnology Letters | 2002年 / 24卷

关键词：

cDNA sequence; defibrase; expression; separation; thrombin-like enzyme;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The total RNA was extracted from the venom gland of snake, Gloydius shedaoensis. The defibrase cDNA was amplified by RT-PCR. Assaying the nucleotide sequence of the cDNA allowed the complete amino acid sequence for defibrase of Gloydius shedaoensis to be determined. The defibrase cDNA was inserted into the vector, pPIC 9K, and expressed in Pichia pastoris which then produced 10 mg target protein l−1.

引用

页码：135 / 138

页数：3

共 54 条

[1]

Au LC(1993)Molecular cloning and sequence analysis of the cDNA for ancrod, a thrombin-like enzyme from the venom of Biochem. J. 294 387-390

[2]

Lin SB(1999)Cloning, sequence analysis and expression in Biochem. Mol. Biol. Int. 47 217-225

[3]

Chou JS(2000). Protein Expr. Purif. 19 41-47

[4]

Teh GW(1981) of the cDNA of the thrombinlike enzyme (Pallabin) from the venom of Meth. Enzymol. 80 341-361

[5]

Chang KJ(2000)Expression, refolding, and activity of a recombinant nonhemorrhagic snake venom metalloprotease Toxicon 38 1225-1236

[6]

Shih CM(1991)Assay of coagulation protease using peptide chromogenic and fluorogenic substrates J. Biochem. 109 632-637

[7]

Fan CY(2000)Characterization of a thrombin-like enzyme from the venom of Biochim. Biophys. Acta 1477 146-156

[8]

Qian YC(1999)Expression of cDNA for Batroxobin, a thrombin-like snake venom enzyme Biochem. Biophys. Res. Commun. 255 412-415

[9]

Yang SL(1990)Snake venom protease affecting hemostasis and thrombosis Adv. Exp. Med. Biol. 281 165-175

[10]

Gong Y(1985)cDNA clonging and expression of Acutin, a thrombin-like enzyme from Anal. Biochem. 150 76-85

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