Non-random inheritance of mitochondrial genomes in Citrus hybrids produced by protoplast fusion

被引：6

作者：

Cabasson C.M. ^{[1
]}

Luro F. ^{[2
]}

Ollitrault P. ^{[1
]}

Grosser J.W. ^{[3
]}

机构：

[1] CIRAD-FLHOR, 34032 Montpellier

[2] Station de Recherches Agronomiques, INRA-CIRAD, 20230 San Nicolao, Corsica

[3] Citrus Research and Education Center, University of Florida, IFAS, Lake Alfred, FL 33850

来源：

Plant Cell Reports | 2001年 / 20卷 / 07期

关键词：

Citrus; Mitochondrial DNA; Nuclear DNA; Protoplast fusion; RFLP;

D O I：

10.1007/s002990100370

中图分类号：

学科分类号：

摘要：

Somatic hybridization offers the possibility of manipulating chloroplast and mitochondrial genomes and evaluating their role on cultivar qualities in citrus. Numerous associations between Willow-leaf mandarin (Citrus deliciosa Ten.), as embryogenic parent, and sweet orange cv. Valencia (Citrus sinensis (L.) Osb.), as mesophyll parent, and between Willow-leaf mandarin (embryogenic parent) and grapefruit cv. Duncan (Citrus paradisi Macf.) (mesophyll parent) were obtained by the fusion of protoplasts induced by polyethylene glycol. Regenerated plants were characterized by flow cytometry and nuclear and mitochondrial DNA restriction fragment length polymorphism (RFLP). All plants were diploid. Diploid plants with the nuclear RFLP patterns of mandarin or sweet orange were identified in the progeny between these two parents, while only grapefruit nuclear types were found in the mandarin + grapefruit progeny. The diploid plants with the nuclear profile of the mesophyll parent originated systematically from cells formed through spontaneous association of the nuclear genome of the mesophyll parent and the mitochondrial genome of the embryogenic parent. These plants are assumed to be alloplasmic hybrids or cybrids. They were viable and have been propagated for field testing.

引用

页码：604 / 609

页数：5

共 31 条

[1]

Bonen L., Boer P.H., Gray M.W., The wheat cytochrome oxidase subunit II gene has an intron insert and tree radical amino acid changes relative to maize, EMBO J, 3, pp. 2531-2536, (1984)

[2]

Bonen L., Boer P.H., McIntosh J.E., Gray M.W., Nucleotide sequence of the wheat mitochondrial gene for subunit I of cytochrome oxidase, Nucleic Acids Res, 15, (1987)

[3]

Bonnema A.B., Melzer J.M., Murray L.W., O'Connell M.A., Non-random inheritance of organellar genomes in symmetric and asymmetric somatic hybrids between Lycopersicon esculentum and L. pennellii, Theor Appl Genet, 84, pp. 435-442, (1992)

[4]

Cabasson C., Ollitrault P., Cote F.-X., Michaux-Ferriere N., Dambier D., Dalnic R., Teisson C., Characteristics of Citrus cells cultures during undifferentiated growth on sucrose and somatic embryogenesis on galactose, Physiol Plant, 93, pp. 464-470, (1995)

[5]

Dawson A.J., Jones V.P., Leaver C.J., The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site, EMBO J, 3, pp. 2107-2113, (1984)

[6]

Delseny M., Cooke R., Penon P., Sequence heterogenecity in radish nuclear ribosomal RNA genes, Plant Sci Lett, 30, pp. 107-119, (1983)

[7]

Dewey R.E., Levings C.S., Timothy D.H., Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria, Plant Physiol, 79, pp. 914-919, (1985)

[8]

Doyle J.J., Doyle J.L., A rapid DNA isolation procedure for small quantities of fresh leaf tissue, Phytochem Bull, 19, pp. 11-15, (1987)

[9]

Falconet D., Delorme S., Lejeune B., Sevignac M., Delcher E., Bazetoux S., Quetier F., Wheat mitochondrial 26S ribosomal RNA gene has no intron and is present in multiple copies arising by recombination, Curr Genet, 9, pp. 169-174, (1985)

[10]

Grosser J.W., Gmitter F.G., Protoplast fusion and citrus improvement, Plant Breed Rev, 8, pp. 339-374, (1990)

← 1 2 3 4 →