AFLP genetic polymorphism in wild barley (Hordeum spontaneum) populations in Israel

被引:55
作者
T. Turpeinen
T. Vanhala
E. Nevo
E. Nissilä
机构
[1] MTT Agrifood Research Finland,
[2] Plant Production Research,undefined
[3] Crops and Biotechnology,undefined
[4] FIN-31600 Jokioinen,undefined
[5] Finland,undefined
[6] Laboratory of Plant Breeding,undefined
[7] Department of Plant Sciences,undefined
[8] Wageningen University,undefined
[9] P.O.Box 386,undefined
[10] 6700 AJ Wageningen,undefined
[11] Netherlands,undefined
[12] Institute of Evolution,undefined
[13] University of Haifa,undefined
[14] Mt. Carmel,undefined
[15] Haifa 31905,undefined
[16] Israel,undefined
[17] Boreal Plant Breeding Ltd,undefined
[18] FIN-31600 Jokioinen,undefined
[19] Finland,undefined
关键词
Wild barley; Amplified fragment length polymorphism; AFLP; Genetic diversity;
D O I
10.1007/s00122-002-1151-6
中图分类号
学科分类号
摘要
The genetic diversity produced by the amplified fragment length polymorphism (AFLP) method was studied in 94 genotypes of wild barley, Hordeum spontaneum (C. Koch) Thell., originating from ten ecologically and geographically different locations in Israel. Eight primer pairs produced 204 discernible loci of which 189 (93%) were polymorphic. Each genotype had a unique banding profile and the genetic similarity coefficient varied between 0.74 and 0.98. The phenogram generated from these similarities by the UPGMA method did not group genotypes strictly according to their geographical origin, which pattern was also seen in the principal coordinate (PCO) plot. Genetic diversity was larger within (69%) than among (31%) populations. Associations between ecogeographical variables and the mean gene diversity were found at one primer pair. The results are discussed and compared with data obtained by the simple sequence repeat (SSR) method.
引用
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页码:1333 / 1339
页数:6
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