A new triterpenoid from Panax ginseng exhibits cytotoxicity through p53 and the caspase signaling pathway in the HepG2 cell line

被引：11

作者：

Huang J. ^{[1
,2
]}

Tang X.-H. ^{[1
]}

Ikejima T. ^{[2
]}

Sun X.-J. ^{[3
]}

Wang X.-B. ^{[4
]}

Xi R.-G. ^{[4
]}

Wu L.-J. ^{[1
,5
]}

机构：

[1] Department of Phytochemistry, Shenyang Pharmaceutical University

[2] China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016

[3] School of Chemistry, Qiqihaer University, Qiqihaer 161006

[4] School of Pharmacy, Department of Traditional Chinese Medicine, 210th Hospital of People Liberation Army

[5] Department of Phytochemistry, Shenyang Pharmaceutical University, Shenyang 110016, 49#

来源：

Archives of Pharmacal Research | 2008年 / 31卷 / 3期

关键词：

Caspase; Cell cycle; Cytotoxicity; HepG2; p53; Panax ginseng; Triterpenoid;

D O I：

10.1007/s12272-001-1159-8

中图分类号：

学科分类号：

摘要：

A new triterpenoid, 20(R),22(ξ),24(S)-dammar-25(26)-ene-3β, 6α,12β,20,22,24-hexanol (1), and three known triterpenoids, β-D-glucopyranoside,(3β,12β)-12,20-dihydroxydammar-24-en-3-yl, 6-acetate (2), 20(R)-ginsenoside Rg3 (3), and 20(R)-ginsenoside Rh2 (4), were isolated from the leaves of Panax ginseng. Their structures were determined by chemical analysis and spectral methods (IR, 1D and 2D NMR, HR-ESI-MS). Compounds 1-4 were exhibited various degrees of cytotoxicity in the human hepatoma cell line, HepG2. Compound 1 had the highest cytotoxic potency, with an IC50 value of 20.1 μM, by stimulating p53-mediated cell cycle arrest at the G1 to S phase transition, leading to apoptosis via activation of the caspase signaling pathway. © 2008 The Pharmaceutical Society of Korea.

引用

页码：323 / 329

页数：6

共 26 条

[1]

Bunz F., Dutriaux A., Lengauer C., Waldman T., Zhou S., Brown J.P., Sedivy J.M., Kinzler K.W., Vogeistein B., Requirement for p53 and p21 to sustain G2 arrest after DNA damage, Science, 282, pp. 1497-1501, (1998)

[2]

Chen Y.J., Su S.X., Ma Q.F., Pei Y.P., Yao X.S., Studies on new minor saponins isolated from leaves of Panax ginseng C. A. Meyer, Acta Pharmaceutica Sinica, 22, pp. 685-689, (1987)

[3]

Ciciarello M., Mangiacasale R., Casenghi M., Limongi M.Z., D'Angelo M., Soddu S., Lavia P., Cundari E., P53 displacement from centrosomes and p53-mediated G1 arrest following transient inhibition of the mitotic spindle, J. Biol. Chem., 276, pp. 19205-19213, (2001)

[4]

Dou D.Q., Chen Y.J., Liang L.H., Pang F.G., Shimizu N., Takeda T., Six new dammarane-type triterpene saponins from the leaves of Panax ginseng, Chem. Pharm. Bull., 49, pp. 442-446, (2001)

[5]

Feng B.S., Wang X.B., Wang D.Q., Studies on dammarane-type triterpene saponins from the leaves of Panax japonicus C.A. Meyer var. major (Burk.) C.Y.Wu et Feng collected in Qinling, Acta Bot Yunnan, 9, pp. 477-484, (1987)

[6]

Haupt S., Berger M., Goldberg Z., Haupt Y., Apoptosis-the p53 network, J. Cell. Sci., 116, pp. 4077-4085, (2003)

[7]

Hill P.A., Tumber A., Meikle M.C., Multiple extracellular signals promote osteoblast survival and apoptosis, Endocrinology, 138, pp. 3849-3858, (1997)

[8]

Hu H.B., Ahn N.S., Yang X.L., Lee Y.S., Kang K.S., Ganoderma lucidum extract induces cell cycle arrest and apoptosis in MCF-7 human breast cancer cell, Int. J. Cancer, 102, pp. 250-253, (2002)

[9]

Kastan M.B., Onyekwere O., Sidransky D., Vogelstein B., Craig R.W., Participation of p53 protein in the cellular response to DNA damage, Cancer Res., 51, pp. 6304-6311, (1991)

[10]

Kawazoe N., Watabe M., Masuda Y., Nakajo S., Nakaya K., Tiamil is involved in the regulation of bufalin-induced apoptosis in human leukemia cells, Oncogene, 18, pp. 2413-2421, (1999)

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