Cloning and expression analysis of cDNAs corresponding to genes activated in cucumber showing systemic acquired resistance after BTH treatment

被引：27

作者：

Bovie C. ^{[1
]}

Ongena M. ^{[2
]}

Thonart P. ^{[2
]}

Dommes J. ^{[1
]}

机构：

[1] Lab. Biol. Molec. de Biotech. Veg., Dept. des Sciences de la Vie, Université de Liège

[2] Ctr. Wallon de Biologie Industrielle, Unité de Bioindustries, Fac.́ Univ. des Sci. Agronom.

来源：

BMC Plant Biology | / 4卷 / 1期

关键词：

Salicylic Acid; Cucumber Plant; Induce Systemic Resistance; Salicylic Acid Accumulation; Tobacco Necrosis Virus;

D O I：

10.1186/1471-2229-4-15

中图分类号：

学科分类号：

摘要：

Background: Infection of plants by necrotizing pathogens can lead to the rapid and localized induction of a complex set of defense responses resulting in a restriction of pathogen growth and spread. Subsequently, an increase of plant resistance against a broad spectrum of pathogens is observed systemically. This plant immunity is known as Systemic Acquired Resistance. To identify components of the transduction pathway, we cloned and analysed the expression pattern of several mRNAs accumulating in cucumber plants after induction of Systemic Acquired Resistance. Results: We tested on cucumber different compounds known to induce systemic acquired resistance. Among these, BTH (benzo(1,2,3)thiadiazole- 7-carbothioic acid S-methyl ester) proved to be very effective. mRNA RT-PCR differential display was used to identify mRNA sequences induced 24 hours after the application of 10 μM BTH to cucumber plants. A cDNA library constructed from cucumber plants sprayed with 10 μM BTH was screened to get corresponding full length cDNAs. Among the identified cDNAs were those coding for a putative ras-related GTP-binding protein, a putative beta-1,4-N- Acetylglucosaminyltranferase III and a putative pathogenesis related protein. The time course of accumulation of the three corresponding mRNAs was analysed by northern blotting in plants treated by BTH or in plants infected by Colletotrichum lagenarium. Conclusions: The mRNA RT-PCR differential display technique allowed the identification of three genes possibly involved in Systemic Acquired Resistance in cucumber. Pathogenesis-related proteins are known to be involved in plant defence against pathogens. GTP-binding protein and N-acetylglucosaminyltranferase III have been reported to be components of signal transduction pathways in mammals and plants. © 2004 Bovie et al; licensee BioMed Central Ltd.

引用

共 52 条

[1]

Heath M.C., Hypersensitive response related death, Plant Mol Biol, 44, pp. 321-334, (2000)

[2]

Ward E.R., Uknes S.J., Wlilliams S.C., Dincher S.S., Wiederhold D.L., Alexander D.C., Ahl-Goy P., Metraux J.-P., Ryals J.A., Coordinate gene activity in response to agents that induce systemic acquired resistance, Plant Cell, 3, pp. 1085-1094, (1991)

[3]

Klessig D.F., Malamy J., The salicylic acid signal in plants, Plant Mol Biol, 26, pp. 1439-1458, (1994)

[4]

Metraux J.-P., Signer H., Ryals J., Ward E., Wyss-Benz M., Gaudin J., Rascdorf K., Schmid E., Blum W., Inverardi B., Increase in salicylic acid at the onset of systemic acquired resistance in cucumber, Science, 250, pp. 1004-1006, (1990)

[5]

Malamy J., Carr J.P., Klessig D.F., Raskin I., Salicylic acid: A likely endogenous signal in the resistance response of tobacco to viral infection, Science, 250, pp. 1002-1004, (1990)

[6]

Uknes S., Winter A.M., Delaney T., Vernooij B., Morse A., Friedrich L., Nye G., Potter S., Ward E., Ryals J., Biological induction of systemic acquired resistance in Arabidopsis, Mol Plant-Microbe Interact, 6, pp. 692-698, (1993)

[7]

Delaney T.P., Uknes S., Vernoij B., Friedrich L., Weymann K., Negrotto D., Gaffney T., Gur-Rella M., Kessmann H., Ward E., Ryals J., A central role of salicylic acid in plant disease resistance, Science, 266, pp. 1247-1250, (1994)

[8]

Gaffney T., Friedrich L., Vernooij B., Negrotto D., Nye G., Uknes S., Ward E., Kessmann H., Ryals J., Requirement of salicylic acid for the induction of systemic acquired resistance, Science, 261, pp. 754-756, (1993)

[9]

Cao H., Bowling S.A., Gordon S., Dong X., Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance, Plant Cell, 6, pp. 1583-1592, (1994)

[10]

Delaney T.P., Friedrich L., Ryals J.A., Arabidopsis signal transduction mutant defective in chemically and biologically induced disease resistance, Proc Natl Acad Sci U S A, 92, pp. 6602-6606, (1995)

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