A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen

被引：29

作者：

Escobar R.H. ^{[1
]}

Mafla G. ^{[1
]}

Roca W.M. ^{[1
]}

机构：

[1] Biotechnology Research Unit, Ctro. Internacional de Agric. Trop., A. A. 6713, Cali

来源：

Plant Cell Reports | 1997年 / 16卷 / 7期

关键词：

Cassava (Manihot esculenta Crantz); Cryopreservation;

D O I：

10.1007/BF01092769

中图分类号：

学科分类号：

摘要：

Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen, the shoot tips were removed, rapidly warmed, and recultured sequentially in three recovery media. After 2 weeks, the regeneration of frozen shoot tips was completed. Genotypes with a low response were identified. Their response was attributed to the effects of pre- and post- freezing steps. Refining the methodology led to a consistent 50-70% plant recovery.

引用

页码：474 / 478

页数：4

共 9 条

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