Multiple injury approach and its use for toxicity studies

被引:2
作者
Ara Arutunyan
Luther Swift
Narine Sarvazyan
机构
[1] Texas Tech University Health Sciences Center,Department of Physiology
关键词
Cultured cardiomyocytes; fluorescent indicators; calcium transients; oxidative stress; imaging;
D O I
10.1385/CT:4:1:01
中图分类号
学科分类号
摘要
We present an experimental approach that allows exposure of cells plated on a single coverslip to multiple distinct environments. The original chamber design created a small region of injury using geometrically defined flows of the control and ischemic solutions. Modifications of the original chamber design presented in this article produce a range of flow patterns that can be advantageous for a variety of imaging applications. These applications include: experiments that address effects of different treatments applied to a cell network, parallel testing of negative and positive controls using a single coverslip, border effect studies, evaluation of the treatment’s reversibility, and simultaneous monitoring of a cell layer loaded with different fluorescent indicators. The method also can be used to reveal both micro- and macroscopic features of propagation, conduction, and cell coupling in a normal or altered cardia cell network. These possibilities are illustrated in cultures of neonatal rat cardiomyocytes using oxidant-and calcium-sensitive fluorescent indicators.
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页码:1 / 10
页数:9
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