A single band does not always represent single bacterial strains in denaturing gradient gel electrophoresis analysis

被引：1

作者：

Hiroyuki Sekiguchi

Noriko Tomioka

Tadaatsu Nakahara

Hiroo Uchiyama

机构：

[1] National Institute for Environmental Studies,Institute of Applied Biochemistry

[2] University of Tsukuba,undefined

来源：

Biotechnology Letters | 2001年 / 23卷

关键词：

bias; DGGE; microbial community; PCR; 16S rDNA;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

DNA in a denaturing gradient gel electrophoresis (DGGE) band that could not be sequenced after recovery from the gel was cloned into a TA cloning vector and a library was constructed and then 13 clones randomly picked up from the library was sequenced. Although the excised DNA from the DGGE gel showed a single band, the library consisted of several different sequences phylogenetically. This phenomenon was also observed in several other DGGE bands. Therefore, this suggests that a single DGGE band does not always represent a single bacterial strain and a new bias for quantitative analyses based on band intensities has been identified.

引用

页码：1205 / 1208

页数：3

共 40 条

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